Toward Gene Therapy of Hypertension: Experimental Study on Hypertensive ISIAH Rats Full article
Journal |
Biochemistry (Moscow)
ISSN: 0006-2979 , E-ISSN: 1608-3040 |
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Output data | Year: 2017, Volume: 82, Number: 4, Pages: 454-457 Pages count : 4 DOI: 10.1134/S000629791704006X | ||||||||
Tags | antisense oligonucleotides, hypertension, ISIAH rats, nanocomposites | ||||||||
Authors |
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Affiliations |
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Funding (1)
1 | Russian Science Foundation | 16-15-10073 |
Abstract:
TiO2-based nanocomposites were prepared to deliver oligonucleotides into cells. The nanocomposites were designed by the immobilization of polylysine-containing oligonucleotides on TiO2-nanoparticles (TiO2·PL-DNA). We showed for the first time the possibility of using the proposed nanocomposites for treatment of hypertensive disease by introducing them into hypertensive ISIAH rats developed as a model of stress-sensitive arterial hypertension. The mRNA of the gene encoding angiotensin I-converting enzyme (ACE1) involved in the synthesis of angiotensin II was chosen as a target. Administration (intraperitoneal injection and inhalation) of the nanocomposite showed a significant (by 20-30 mm Hg) decrease in systolic blood pressure when the nanocomposite contained the ACE1 gene-targeted oligonucleotide. When using the oligonucleotide with a random sequence, no effect was observed. Further development and improvement of the inhalation nanocomposite drug delivery to systemic hypertensive disease treatment promises new possibilities for clinical practice.
Cite:
Repkova M.N.
, Levina A.S.
, Seryapina A.A.
, Shikina N.V.
, Bessudnova E.V.
, Zarytova V.F.
, Markel A.L.
Toward Gene Therapy of Hypertension: Experimental Study on Hypertensive ISIAH Rats
Biochemistry (Moscow). 2017. V.82. N4. P.454-457. DOI: 10.1134/S000629791704006X WOS Scopus РИНЦ
Toward Gene Therapy of Hypertension: Experimental Study on Hypertensive ISIAH Rats
Biochemistry (Moscow). 2017. V.82. N4. P.454-457. DOI: 10.1134/S000629791704006X WOS Scopus РИНЦ
Original:
Репкова М.Н.
, Левина А.С.
, Серяпина А.А.
, Шикина Н.В.
, Бессуднова Е.В.
, Зарытова В.Ф.
, Маркель А.Л.
На пути к генотерапии гипертонической болезни: экспериментальное исследование на гипертензивных крысах линии НИСАГ (ISIAH)
Биохимия. 2017. Т.82. №4. С.620-625. RSCI РИНЦ
На пути к генотерапии гипертонической болезни: экспериментальное исследование на гипертензивных крысах линии НИСАГ (ISIAH)
Биохимия. 2017. Т.82. №4. С.620-625. RSCI РИНЦ
Dates:
Submitted: | Sep 27, 2016 |
Published print: | Apr 1, 2017 |
Published online: | Apr 18, 2017 |
Identifiers:
Web of science | WOS:000399430800006 |
Scopus | 2-s2.0-85017514264 |
Elibrary | 29500520 |
Chemical Abstracts | 2017:623026 |
OpenAlex | W2607495317 |