DNA, Oligosaccharides, and Mononucleotides Stimulate Oligomerization of Human Lactoferrin Full article
Journal |
Journal of Molecular Recognition
ISSN: 0952-3499 , E-ISSN: 1099-1352 |
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Output data | Year: 2009, Volume: 22, Number: 4, Pages: 330-342 Pages count : 11 DOI: 10.1002/jmr.952 | ||||
Tags | Atp, Dna, Human lactoferrin, Influence on rate and degree of oligomerization | ||||
Authors |
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Affiliations |
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Funding (4)
1 | Президиум РАН | 10.5 |
2 | Президиум РАН | 11.8 |
3 | Civilian Research and Development Foundation | Y5-B-08-11 |
4 | The Ministry of Education and Science of the Russian Federation | 2.2.2.3.16036 |
Abstract:
Using small-angle X-ray scattering (SAXS), light scattering (LS), and soft laser ablation we have shown that lactoferrin (LF) in solution at neutral pH is oligomerized in the absence of salt or at physiological salt concentrations. The level of oligomerization depends on the concentration of LF, KCl or NaCl, and on the duration of the protein storage in solution. At the concentrations comparable with those in human milk (1 - 6 mg/ml), the average radius of gyration (Rg) values of LF can attain 400 - 480 A˚, while fresh solution of previously lyophylized LF demonstrate a lower average Rg (50 - 100 A˚), and Rg value characterizing the LF monomer formed at 1 M NaCl is 26.7 A˚. The addition of oligonucleotides, oligosaccharides, or mononucleotides to LF in the presence or in the absence of KCl with different level of initial oligomerization accelerates the oligomerization rate and increases the Rg values up to 600 - 700 A˚, which correspond to associates containing ten or more protein molecules. During gel filtration on Sepharose 4B, high-degree LF oligomers dissociate nearly completely forming different degraded complexes, but in some cases it is
possible to reveal small amount of a decamer. A possible role for oligomerization of LF, a highly polyfunctional protein, for its different biological activities is discussed.
Cite:
Nevinskii A.G.
, Soboleva S.E.
, Tuzikov F.V.
, Buneva V.N.
, Nevinsky G.A.
DNA, Oligosaccharides, and Mononucleotides Stimulate Oligomerization of Human Lactoferrin
Journal of Molecular Recognition. 2009. V.22. N4. P.330-342. DOI: 10.1002/jmr.952 WOS Scopus РИНЦ
DNA, Oligosaccharides, and Mononucleotides Stimulate Oligomerization of Human Lactoferrin
Journal of Molecular Recognition. 2009. V.22. N4. P.330-342. DOI: 10.1002/jmr.952 WOS Scopus РИНЦ
Dates:
Submitted: | Nov 13, 2008 |
Accepted: | Mar 12, 2009 |
Published online: | Apr 21, 2009 |
Published print: | Jul 1, 2009 |
Identifiers:
Web of science | WOS:000267297700008 |
Scopus | 2-s2.0-67650267000 |
Elibrary | 13614027 |
Chemical Abstracts | 2009:775119 |
Chemical Abstracts (print) | 151:486392 |
OpenAlex | W1964772561 |