Delivery of Bleomycin A5 into Cells Using TiO2 Nanoparticles to Enhance the Degradation of Intracellular DNA Full article
Journal |
Nanotechnologies in Russia
ISSN: 1995-0780 , E-ISSN: 1995-0799 |
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Output data | Year: 2013, Volume: 8, Number: 3-4, Pages: 277-282 Pages count : 6 DOI: 10.1134/S1995078013020134 | ||||||
Tags | Bleomycin A5 (Blm), fluorescein residue (Flu), fluoresceinisothiocyanate (FITC), titanium dioxide nanoparticles (TiO2) | ||||||
Authors |
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Affiliations |
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Funding (1)
1 | Russian Foundation for Basic Research | 11-04-01408 (01201158854) |
Abstract:
Different types of bleomycins are used extensively in cancer therapy. Antibiotics scarcely penetrate through cell membranes, leading to the necessity of using high concentrations of the drug and, therefore, overall body toxicity. To study the penetration of an antibiotic into cells, the fluorescein residue (Flu) was introduced in bleomycin A5 (Blm) to form BlmFlu. It was shown by confocal fluorescent microscopy that BlmFlu in the presence of titanium dioxide nanoparticles (TiO2, 3–5 nm), in contrast to free BlmFlu, efficiently penetrate the cell cytoplasm. After being delivered into cells in the presence of TiO2, Blm appeared to be 7–10 times more efficient than free Blm in the fragmentation of intracellular nucleic acids. The results suggest that nanoparticles are very promising to promote the delivery of antibiotics into cells, which will allow reducing their therapeutic dose.
Cite:
Shatskaya N.V.
, Levina A.S.
, Repkova M.N.
, Baiborodin S.I.
, Shikina N.V.
, Ismagilov Z.R.
, Zarytova V.F.
Delivery of Bleomycin A5 into Cells Using TiO2 Nanoparticles to Enhance the Degradation of Intracellular DNA
Nanotechnologies in Russia. 2013. V.8. N3-4. P.277-282. DOI: 10.1134/S1995078013020134 Scopus РИНЦ
Delivery of Bleomycin A5 into Cells Using TiO2 Nanoparticles to Enhance the Degradation of Intracellular DNA
Nanotechnologies in Russia. 2013. V.8. N3-4. P.277-282. DOI: 10.1134/S1995078013020134 Scopus РИНЦ
Original:
Шацкая Н.В.
, Левина А.С.
, Репкова М.Н.
, Байбородин С.И.
, Шикина Н.В.
, Исмагилов З.Р.
, Зарытова В.Ф.
Доставка блеомицина А5 в клетки с помощью TiO2 наночастиц для усиления деструкции внутриклеточных ДНК
Российские нанотехнологии. 2013. Т.8. №3-4. С.108-112. РИНЦ
Доставка блеомицина А5 в клетки с помощью TiO2 наночастиц для усиления деструкции внутриклеточных ДНК
Российские нанотехнологии. 2013. Т.8. №3-4. С.108-112. РИНЦ
Dates:
Submitted: | Jul 25, 2012 |
Accepted: | Dec 11, 2012 |
Published print: | Mar 1, 2013 |
Published online: | May 10, 2013 |
Identifiers:
Scopus | 2-s2.0-84877621586 |
Elibrary | 20427870 |
OpenAlex | W2000371402 |