Knockdown of Different Influenza A Virus Subtypes in Cell Culture by a Single Antisense Oligodeoxyribonucleotide Full article
Journal |
International Journal of Antimicrobial Agents
ISSN: 0924-8579 , E-ISSN: 1872-7913 |
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Output data | Year: 2015, Volume: 46, Number: 1, Pages: 125-128 Pages count : 4 DOI: 10.1016/j.ijantimicag.2015.03.004 | ||||||
Tags | DNA fragments, Influenza A virus, Nanocomposite, TiO2 nanoparticles | ||||||
Authors |
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Affiliations |
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Funding (3)
1 | Russian Foundation for Basic Research | 14-04-00753 |
2 | Russian Foundation for Basic Research | 15-04-04109 |
3 | The Ministry of Education and Science of the Russian Federation | 16.512.11.2267 (01201176480) |
Abstract:
Influenza is a heavy socially significant viral infection that affects humans, birds, and wild and domestic animals. The threat of existing and new highly pathogenic subtypes of influenza A virus (IAV) makes it necessary to develop an effective drug that may affect different IAV strains. For this purpose, oligodeoxynucleotides (DNA fragments) attached to titanium dioxide (TiO2) nanoparticles through a polylysine linker, forming TiO2·PL–DNA nanocomposites, that penetrated into cells without transfection agents were used. For the first time, efficient (≥99.9%) suppression of the reproduction of different subtypes of IAV, including highly pathogenic H5N1 and H1N1, was achieved. These results were obtained using the TiO2·PL–DNA nanocomposite bearing a single antisense oligodeoxynucleotide (0.1 μM) targeted to the conserved 3′-noncoding region of RNA segment 5, which is common to all tested strains. Very efficient suppression of the reproduction of different subtypes of IAV was probably achieved due to the use of the proposed delivery system for oligonucleotides in the form of the TiO2·PL–DNA nanocomposites. These results indicate the possibility of creating an efficient drug to affect existing and newly emerging pathogenic IAV strains
Cite:
Levina A.S.
, Repkova M.N.
, Mazurkova N.A.
, Makarevich E.V.
, Ismagilov Z.R.
, Zarytova V.F.
Knockdown of Different Influenza A Virus Subtypes in Cell Culture by a Single Antisense Oligodeoxyribonucleotide
International Journal of Antimicrobial Agents. 2015. V.46. N1. P.125-128. DOI: 10.1016/j.ijantimicag.2015.03.004 WOS Scopus РИНЦ
Knockdown of Different Influenza A Virus Subtypes in Cell Culture by a Single Antisense Oligodeoxyribonucleotide
International Journal of Antimicrobial Agents. 2015. V.46. N1. P.125-128. DOI: 10.1016/j.ijantimicag.2015.03.004 WOS Scopus РИНЦ
Dates:
Submitted: | Oct 21, 2014 |
Accepted: | Mar 6, 2015 |
Published online: | Apr 20, 2015 |
Published print: | Jul 1, 2015 |
Identifiers:
Web of science | WOS:000355681000021 |
Scopus | 2-s2.0-84934910294 |
Elibrary | 23628284 |
Chemical Abstracts | 2015:723202 |
Chemical Abstracts (print) | 163:111528 |
OpenAlex | W2129949745 |