High-Performance Method for Specific Effect on Nucleic Acids in Cells Using TiO2~DNA Nanocomposites Научная публикация
Журнал |
Scientific Reports
, E-ISSN: 2045-2322 |
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Вых. Данные | Год: 2012, Том: 2, Страницы: 756 Страниц : 6 DOI: 10.1038/srep00756 | ||||||||
Ключевые слова | TITANIUM-DIOXIDE NANOPARTICLES; INFLUENZA-A VIRUS; ANTISENSE OLIGONUCLEOTIDES; RNA INTERFERENCE; DRUG-DELIVERY; THERAPEUTICS; REPLICATION; INHIBITION; TOXICITY; VECTORS | ||||||||
Авторы |
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Организации |
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Информация о финансировании (3)
1 | Российский фонд фундаментальных исследований | 11-04-01408 (01201158854) |
2 | Министерство образования и науки Российской Федерации | 16.512.11.2267 (01201176480) |
3 | Сибирское отделение Российской академии наук | 61 |
Реферат:
Nanoparticles are used to solve the current drug delivery problem. We present a high-performance method for efficient and selective action on nucleic acid target in cells using unique TiO2 center dot PL-DNA nanocomposites (polylysine-containing DNA fragments noncovalently immobilized onto TiO2 nanoparticles capable of transferring DNA). These nanocomposites were used for inhibition of human influenza A (H3N2) virus replication in infected MDCK cells. They showed a low toxicity (TC50 approximate to 1800 mu g/ml) and a high antiviral activity (>99.9% inhibition of the virus replication). The specificity factor (antisense effect) appeared to depend on the delivery system of DNA fragments. This factor for nanocomposites is ten-times higher than for DNA in the presence of lipofectamine. IC50 for nanocomposites was estimated to be 1.5 mu g/ml (30nM for DNA), so its selectivity index was calculated as similar to 1200. Thus, the proposed nanocomposites are prospective for therapeutic application.
Библиографическая ссылка:
Levina A.S.
, Repkova M.N.
, Ismagilov Z.R.
, Shikina N.V.
, Malygin E.G.
, Mazurkova N.A.
, Zinovʹev V.V.
, Evdokimov A.A.
, Baiborodin S.I.
, Zarytova V.F.
High-Performance Method for Specific Effect on Nucleic Acids in Cells Using TiO2~DNA Nanocomposites
Scientific Reports. 2012. V.2. P.756. DOI: 10.1038/srep00756 WOS Scopus РИНЦ
High-Performance Method for Specific Effect on Nucleic Acids in Cells Using TiO2~DNA Nanocomposites
Scientific Reports. 2012. V.2. P.756. DOI: 10.1038/srep00756 WOS Scopus РИНЦ
Файлы:
Полный текст от издателя
Даты:
Поступила в редакцию: | 20 июл. 2012 г. |
Принята к публикации: | 27 сент. 2012 г. |
Опубликована online: | 22 окт. 2012 г. |
Опубликована в печати: | 1 дек. 2012 г. |
Идентификаторы БД:
Web of science | WOS:000310449200002 |
Scopus | 2-s2.0-84868297505 |
РИНЦ | 20498444 |
Chemical Abstracts | 2012:1612669 |
Chemical Abstracts (print) | 159:416669 |
OpenAlex | W2086156791 |