Deciphering How Pore Formation Causes Strain-Induced Membrane Lysis of Lipid Vesicles Научная публикация
Журнал |
Journal of the American Chemical Society
ISSN: 0002-7863 , E-ISSN: 1520-5126 |
||||||||
---|---|---|---|---|---|---|---|---|---|
Вых. Данные | Год: 2016, Том: 138, Номер: 4, Страницы: 1406-1413 Страниц : 8 DOI: 10.1021/jacs.5b12491 | ||||||||
Ключевые слова | Peptides; Polypeptides; Pore size; Viruses | ||||||||
Авторы |
|
||||||||
Организации |
|
Информация о финансировании (2)
1 | National Research Foundation of Singapore | NRF-NRFF2011-01 |
2 | National Medical Research Council | NMRC/CBRG/0005/2012 |
Реферат:
Pore formation by membrane-active antimicrobial peptides is a classic strategy of pathogen inactivation through disruption of membrane biochemical gradients. It remains unknown why some membrane-active peptides also inhibit enveloped viruses, which do not depend on biochemical gradients. Here, we employ a label-free biosensing approach based on simultaneous quartz crystal microbalance-dissipation and ellipsometry measurements in order to investigate how a pore-forming, virucidal peptide destabilizes lipid vesicles in a surface-based experimental configuration. A key advantage of the approach is that it enables direct kinetic measurement of the surface-bound peptide-to-lipid (P:L) ratio. Comprehensive experiments involving different bulk peptide concentrations and biologically relevant membrane compositions support a unified model that membrane lysis occurs at or above a critical P:L ratio, which is at least several-fold greater than the value corresponding to the onset of pore formation. That is consistent with peptide-induced pores causing additional membrane strain that leads to lysis of highly curved membranes. Collectively, the work presents a new model that describes how peptide-induced pores may destabilize lipid membranes through a membrane strain-related lytic process, and this knowledge has important implications for the design and application of membrane-active peptides. © 2016 American Chemical Society.
Библиографическая ссылка:
Jackman J.A.
, Goh H.Z.
, Zhdanov V.P.
, Knoll W.
, Cho N-J.
Deciphering How Pore Formation Causes Strain-Induced Membrane Lysis of Lipid Vesicles
Journal of the American Chemical Society. 2016. V.138. N4. P.1406-1413. DOI: 10.1021/jacs.5b12491 WOS Scopus РИНЦ
Deciphering How Pore Formation Causes Strain-Induced Membrane Lysis of Lipid Vesicles
Journal of the American Chemical Society. 2016. V.138. N4. P.1406-1413. DOI: 10.1021/jacs.5b12491 WOS Scopus РИНЦ
Даты:
Поступила в редакцию: | 2 дек. 2015 г. |
Опубликована online: | 21 янв. 2016 г. |
Опубликована в печати: | 3 февр. 2016 г. |
Идентификаторы БД:
Web of science | WOS:000369558000046 |
Scopus | 2-s2.0-84957538645 |
РИНЦ | 26952160 |
Chemical Abstracts | 2016:67340 |
Chemical Abstracts (print) | 164:196340 |
PMID (PubMed) | 26751083 |
OpenAlex | W2254198722 |