Simulation of Two-Dimensional Streptavidin Crystallization Full article
Journal |
Proteins: Structure, Function and Genetics
ISSN: 0887-3585 , E-ISSN: 1097-0134 |
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Output data | Year: 2001, Volume: 43, Number: 4, Pages: 489-498 Pages count : 10 DOI: 10.1002/prot.1060 | ||||
Tags | Anisotropic islands, Biotinylated lipid layer, Lattice model, Monte Carlo simulations, Protein adsorption, Protein-protein lateral interactions, Streptavidin | ||||
Authors |
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Affiliations |
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Funding (2)
1 | Swedish Research Council | 251-98-796 |
2 | Stiftelsen för strategisk forskning | A3 95:1 |
Abstract:
We present lattice Monte Carlo simulations of the growth of streptavidin islands at a biotinylated lipid layer. The model employed takes into account attractive anisotropic lateral interactions between streptavidin tetramers. With a minimal set of interactions, we reproduce the formation of rectangular islands experimentally observed at pH ≥ 9.0. Specifically, we analyze two scenarios of the island growth. First, if streptavidin is rapidly adsorbed at t = 0 (stepwise coverage change without ongoing adsorption), the average linear island size is found to grow according to the Lifshitz–Slyozov law, R ∝ t1/3. Second, if the island growth occurs in parallel with streptavidin adsorption limited by diffusion in the solution, the Lifshitz–Slyozov law is also applicable, but only at the late stage, when the streptavidin coverage is appreciable.
Cite:
Zhdanov V.P.
, Höök F.
, Kasemo B.
Simulation of Two-Dimensional Streptavidin Crystallization
Proteins: Structure, Function and Genetics. 2001. V.43. N4. P.489-498. DOI: 10.1002/prot.1060 WOS Scopus РИНЦ
Simulation of Two-Dimensional Streptavidin Crystallization
Proteins: Structure, Function and Genetics. 2001. V.43. N4. P.489-498. DOI: 10.1002/prot.1060 WOS Scopus РИНЦ
Dates:
Submitted: | Apr 25, 2000 |
Accepted: | Feb 15, 2001 |
Published online: | Apr 19, 2001 |
Published print: | Jun 1, 2001 |
Identifiers:
Web of science | WOS:000168921000014 |
Scopus | 2-s2.0-0035370882 |
Elibrary | 13379067 |
Chemical Abstracts | 2002:45354 |
OpenAlex | W2083439195 |