The Synthesis of Insulin Fragments and the Study of Their Physicochemical and Immune Properties Full article
Journal |
Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620 , E-ISSN: 1608-330X |
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Output data | Year: 1997, Volume: 23, Number: 12, Pages: 843-849 Pages count : 7 | ||||
Tags | Immunochemistry, Insulin, Receptor binding site, Small-angle X-ray scattering, Synthetic peptides | ||||
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Abstract:
A two-chain peptide was predicted as a receptor binding site of insulin on the basis of theoretical conformational analysis. This dimeric peptide, consisting of the C-terminal A18-A21 tetrapeptide of the insulin A-chain and the C-terminal B17-B30 tetradecapeptide of the insulin B-chain connected with a disulfide bridge, was synthesized along with the C-terminal nonadecapeptide. The analysis of the aggregation state of human insulin and the synthesized linear and dimeric peptides was performed by the small-angle X-ray scattering method. Specific antibodies were produced after rabbit immunization with the dimeric peptide-BSA conjugate. The immunochemical identity of the model dimeric peptide and the corresponding fragment of the insulin molecule were shown by immunoenzyme analysis.
Cite:
Panin L.E.
, Tuzikov F.V.
, Poteryaeva O.N.
, Maksyutov A.Z.
, Tuzikova N.A.
, Sabirov A.N.
The Synthesis of Insulin Fragments and the Study of Their Physicochemical and Immune Properties
Russian Journal of Bioorganic Chemistry. 1997. V.23. N12. P.843-849. Scopus РИНЦ
The Synthesis of Insulin Fragments and the Study of Their Physicochemical and Immune Properties
Russian Journal of Bioorganic Chemistry. 1997. V.23. N12. P.843-849. Scopus РИНЦ
Original:
Панин Л.Е.
, Тузиков Ф.В.
, Потеряева О.Н.
, Максютов А.З.
, Тузикова Н.А.
, Сабиров А.Н.
Синтез фрагментов инсулина и изучение их физико-химических и иммунологических свойств
Биоорганическая химия. 1997. Т.23. №12. С.953-960. WOS РИНЦ
Синтез фрагментов инсулина и изучение их физико-химических и иммунологических свойств
Биоорганическая химия. 1997. Т.23. №12. С.953-960. WOS РИНЦ
Dates:
Published print: | Dec 1, 1997 |
Identifiers:
Scopus | 2-s2.0-27544511456 |
Elibrary | 13270136 |
Citing:
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