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Chemical Ribonucleases: 3.1 The Synthesis of Organic Catalysts for the Phosphodiester Bond Hydrolysis on the Basis of Quaternary Salts of 1,4-Diazabicyclo[2.2.2]octane Full article

Общее Language: Английский, Genre: Full article,
Status: Published, Source type: Translated
Journal Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620 , E-ISSN: 1608-330X
Output data Year: 2000, Volume: 26, Number: 11, Pages: 765-773 Pages count : 9 DOI: 10.1007/BF02759630
Tags Artificial ribonucleases, Catalysis, Polycations, RNA cleavage
Authors Konevetz D.A. 1 , Beck I.E. 2 , Silʹnikov V.N. 1 , Zenkova M.A. 1 , Shishkin G.V. 1
Affiliations
1 Institute of Chemical Biology and Fundamental Medicine SB RAS
2 Boreskov Institute of Catalysis SB RAS

Funding (4)

1 Russian Foundation for Basic Research 99-04-49538
2 Russian Foundation for Basic Research 96-15-97732
3 International Association for the Promotion of Co-operation with Scientists from the New Independent States of the Former Soviet Union 96-1418
4 Russian Foundation for Basic Research 98-03-32958

Abstract: On the basis of imidazole and bisquaternary salts of 1,4-diazabicyclo[2.2.2]octane, a number of highly effective catalysts of the nDm series (here, n is the number of positive charges at neutral pH values and m is the digital code of the catalytically active fragment: 1, histamine, and 2, histidine methyl ester) were synthesized for the cleavage of phosphodiester bonds in ribonucleic acids. A general method for the synthesis of chemical ribonucleases was suggested, which helps vary both the number of positive charges in their RNA-binding domain and the catalytic center. By the example of hydrolysis under physiological conditions of the in vitro transcript of tRNA Lys from human mitochondria, it was shown that the RNA cleavage rate with the nDm conjugates increases approximately 30-fold along with the increase in the number of positive charges from two to four.
Cite: Konevetz D.A. , Beck I.E. , Silʹnikov V.N. , Zenkova M.A. , Shishkin G.V.
Chemical Ribonucleases: 3.1 The Synthesis of Organic Catalysts for the Phosphodiester Bond Hydrolysis on the Basis of Quaternary Salts of 1,4-Diazabicyclo[2.2.2]octane
Russian Journal of Bioorganic Chemistry. 2000. V.26. N11. P.765-773. DOI: 10.1007/BF02759630 publication_identifier_short.scopus_identifier_type publication_identifier_short.rinz_identifier_type
ArticleLinkType.TRANSLATED_TO_ORIGINAL: Коневец Д.А. , Бекк И.Э. , Сильников В.Н. , Зенкова М.А. , Шишкин Г.В.
Химические рибонуклеазы. 3. Синтез органических катализаторов гидролиза фосфодиэфирных связей на основе четвертичных солей 1,4-диазабицикло[2.2.2]октана
Биоорганическая химия. 2000. Т.26. №11. С.852-861. publication_identifier_short.wos_identifier_type publication_identifier_short.scopus_identifier_type publication_identifier_short.rinz_identifier_type
Dates:
Submitted: Mar 29, 2000
Published print: Aug 1, 2000
Identifiers:
publication_identifier.scopus_identifier_type 2-s2.0-0141430773
publication_identifier.rinz_identifier_type 13351426
publication_identifier.accession_number_identifier_type 2000:871429
publication_identifier.chemical_accession_number_identifier_type 134:189912
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