Chemical Ribonucleases: 3.1 The Synthesis of Organic Catalysts for the Phosphodiester Bond Hydrolysis on the Basis of Quaternary Salts of 1,4-Diazabicyclo[2.2.2]octane
Full article
| Общее |
Language:
Английский,
Genre:
Full article,
Status:
Published,
Source type:
Translated
|
| Journal |
Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620
, E-ISSN: 1608-330X
|
| Output data |
Year: 2000,
Volume: 26,
Number: 11,
Pages: 765-773
Pages count
: 9
DOI:
10.1007/BF02759630
|
| Tags |
Artificial ribonucleases, Catalysis, Polycations, RNA cleavage |
| Authors |
Konevetz D.A.
1
,
Beck I.E.
2
,
Silʹnikov V.N.
1
,
Zenkova M.A.
1
,
Shishkin G.V.
1
|
| Affiliations |
| 1 |
Institute of Chemical Biology and Fundamental Medicine SB RAS
|
| 2 |
Boreskov Institute of Catalysis SB RAS
|
|
Funding (4)
|
1
|
Russian Foundation for Basic Research
|
99-04-49538
|
|
2
|
Russian Foundation for Basic Research
|
96-15-97732
|
|
3
|
International Association for the Promotion of Co-operation with Scientists from the New Independent States of the Former Soviet Union
|
96-1418
|
|
4
|
Russian Foundation for Basic Research
|
98-03-32958
|
On the basis of imidazole and bisquaternary salts of 1,4-diazabicyclo[2.2.2]octane, a number of
highly effective catalysts of the nDm series (here, n is the number of positive charges at neutral pH values and m is the digital code of the catalytically active fragment: 1, histamine, and 2, histidine methyl ester) were synthesized for the cleavage of phosphodiester bonds in ribonucleic acids. A general method for the synthesis of chemical ribonucleases was suggested, which helps vary both the number of positive charges in their RNA-binding domain and the catalytic center. By the example of hydrolysis under physiological conditions of the in vitro transcript of tRNA Lys from human mitochondria, it was shown that the RNA cleavage rate with the nDm
conjugates increases approximately 30-fold along with the increase in the number of positive charges from two to four.