Lipoprotein Fractions and Subfractions in a Murine Model of Early Atherosclerosis Conference Abstracts
Conference |
19th World Congress on Heart Disease 25-28 Jul 2014 , Boston |
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Journal |
Cardiology
ISSN: 0008-6312 , E-ISSN: 1421-9751 |
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Output data | Year: 2014, Volume: 128, Number: S1, Article number : 380, Pages count : 1 | ||||||||
Authors |
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Affiliations |
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Abstract:
Objective: To evaluate the effect of repeated P-407 treatment on lipoprotein fractions and subfractions, as well as the onset of atherosclerosis, in a mouse model of early atherosclerosis.
Background: Early diagnosis and changes associated with atherosclerosis are important to clinical medicine. Chronic administration of poloxamer 407 (P-407) produces atherosclerosis in mice with significant dislipidemia and damage to heart vessels.
Methods. We induced early atherosclerosis in mice by using repeated P-407 administration (300 mg/kg, twice per week, 30 days) so as to produce a sustained atherogenic serum lipid profile. A small-angle X-ray scattering (SAXS) method was used for the determination of the fractional and subfractional composition of lipoprotein-cholesterol (LP-C) and lipoprotein-triglyceride (LP-TG) fractions and subfractions.
Results. Mice with early development of atherosclerosis revealed significant dyslipidemia, moderately elevated blood pressure, general lipidosis (lipid deposition in liver cells), and contractile-type changes in cardiomyocytes. The steady-state serum total cholesterol and TG were significantly greater for mice repeatedly treated with P-407 than corresponding values observed following a single dose of P-407 and, therefore, serum lipids returned to baseline values more slowly for mice chronically treated with P-407.
The onset of atherosclerosis was characterized by a steady increase in atherogenic subfractions typical of atherosclerosis observed in humans; namely, an increase in low-density LP (LDL), intermediate-density LP (IDL), and very-low-density LP (VLDL, subfractions VLDL3-5, VLDL1-2).
Conclusions: SAXS was useful in revealing the early changes in the LP-C and LP-TG subfractions during the onset of experimental atherosclerosis.
Cite:
Loginova V.M.
, Korolenko T.A.
, Johnston T.
, Tuzikov F.V.
, Korolenko T.P.
, Maiborodin I.N.
, Spiridonov V.K.
Lipoprotein Fractions and Subfractions in a Murine Model of Early Atherosclerosis
Cardiology. 2014. V.128. NS1. 380 :1-1. WOS РИНЦ
Lipoprotein Fractions and Subfractions in a Murine Model of Early Atherosclerosis
Cardiology. 2014. V.128. NS1. 380 :1-1. WOS РИНЦ
Identifiers:
Web of science | WOS:000341933400380 |
Elibrary | 22774147 |
Citing:
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