Small-Angle X-Ray Characterization of the Nucleoprotein Complexes Resulting from DNA-Induced Oligomerization of
HIV-1 Integrase
Full article
| Общее |
Language:
Английский,
Genre:
Full article,
Status:
Published,
Source type:
Original
|
| Journal |
Nucleic Acids Research
ISSN: 0305-1048
, E-ISSN: 1362-4962
|
| Output data |
Year: 2007,
Volume: 35,
Number: 3,
Pages: 975-987
Pages count
: 13
DOI:
10.1093/nar/gkl1111
|
| Authors |
Baranova Svetlana
1
,
Tuzikov Fedor V.
2
,
Zakharova Olga D.
1
,
Tuzikova Natalia A.
2
,
Calmels Christina
3
,
Litvak Simon
3
,
Tarrago-Litvak Laura
3
,
Parissi Vincent
3
,
Nevinsky Georgy A.
1
|
| Affiliations |
| 1 |
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, Lavrentieva Ave. 8, 630090, Russia
|
| 2 |
Institute of Catalysis, Siberian Division of Russian Academy of Sciences, Lavrentyeva Ave. 3, 630090, Russia
|
| 3 |
UMR 5097 CNRS-Universite Victor Segalen Bordeaux 2, 146 rue Leo Saignat, 33076 Bordeaux cedex, France and IFR 66 Bordeaux, France
|
|
Funding (6)
|
1
|
Президиум РАН
|
10.5
|
|
2
|
Russian Foundation for Basic Research
|
03-04-49781
|
|
3
|
Президиум СО РАН
|
|
|
4
|
University of Bordeaux
|
|
|
5
|
French National Centre for Scientific Research
|
|
|
6
|
Agence Nationale de Recherches sur le Sida et les Hépatites Virales
|
|
HIV-1 integrase (IN) catalyses integration of a DNA copy of the viral genome into the host genome. Specific interactions between retroviral IN and long terminal repeats (LTR) are required for this insertion. To characterize quantitatively the influence of the determinants of DNA substrate specificity on the oligomerization status of IN, we used the small-angle X-ray scattering (SAXS) technique. Under certain conditions in the absence of ODNs IN existed only as monomers. IN preincubation with specific ODNs led mainly to formation of dimers, the relative amount of which correlated well with the increase in the enzyme activity in the 3'-processing reaction. Under these conditions, tetramers were scarce. Non-specific ODNs stimulated formation of catalytically inactive dimers and tetramers. Complexes of monomeric, dimeric and tetrameric forms of IN with specific and non-specific ODNs had varying radii of gyration (R-g), suggesting that the specific sequence-dependent formation of IN tetramers can probably occur by dimerization of two dimers of different structure. From our data we can conclude that the DNA-induced oligomerization of HIV-1 IN is probably of importance to provide substrate specificity and to increase the enzyme activity.