Application of the Immobilized Bacterial Recombinant Lipase from Geobacillus stearothermophilus G3 for the Production of Fatty Acid Methyl Esters
Full article
| Общее |
Language:
Английский,
Genre:
Full article,
Status:
Published,
Source type:
Translated
|
| Journal |
Catalysis in Industry
ISSN: 2070-0504
, E-ISSN: 2070-0555
|
| Output data |
Year: 2016,
Volume: 8,
Number: 2,
Pages: 187-193
Pages count
: 7
DOI:
10.1134/S2070050416020082
|
| Tags |
biodiesel fuel, Geobacillus, immobilized lipase, transesterification |
| Authors |
Samoylova Y.V.
1
,
Piligaev A.V.
1
,
Sorokina K.N.
1,2,3
,
Rozanov A.S.
4
,
Peltek S.E.
2
,
Novikov A.A.
4
,
Almyasheva N.R.
4
,
Parmon V.N.
1,3
|
| Affiliations |
| 1 |
Boreskov Institute of Catalysis, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia
|
| 2 |
Federal Research Center Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences,
Novosibirsk, 630090 Russia
|
| 3 |
Novosibirsk State University, Novosibirsk, 630090 Russia
|
| 4 |
Gubkin Russian State University of Oil and Gas, Moscow, 119991 Russia
|
|
Funding (1)
|
1
|
Federal Agency for Scientific Organizations
|
V.46.4.4.
|
The heterogeneous BCL biocatalyst based on the recombinant extracellular lipase from the thermophilic
bacteria Geobacillus stearothermophilus G3 with an activity of 23.6 U.A./g was prepared by covalent
immobilization on aminated silica gel. The effect of the solvent, temperature (30–60°C), methanol : oil
molar ratio (1 : 1 to 9 : 1), and the amounts of water (1–10%) and catalyst (0.25–25%) on the yield of fatty
acid methyl esters (FAME) during the methanolysis of sunflower oil with BCL was studied. The maximum
yield of FAME was 43%. The biocatalyst exhibits high operational stability: after 480 h of operation
(20 cycles), it retains more than 50% of its original activity, making BCL a promising catalyst for application
in manufacturing of FAME as feedstock for biodiesel production.