Interaction of Eukaryotic DNA with Apolipoprotein A-I and Its Complexes with Glucocorticoids Full article
Journal |
Biophysics
ISSN: 0006-3509 , E-ISSN: 1555-6654 |
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Output data | Year: 2000, Volume: 45, Number: 4, Pages: 594-602 Pages count : 9 | ||||
Tags | DNA, APOLIPOPROTEIN A-I, (APOLIPOPROTEIN A-I)-GLUCOCORTICOID COMPLEXES, INTERACTION | ||||
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Abstract:
A biochemically active complex of apobpoprotein A-I with tetrahydrocortisol was revealed and found to stimulate gene expression in hepatocytes. In native DNA isolated from rat liver, single-stranded regions appeared upon titration with a complex (apolipoprotein A-I)-tetrahydrocortisol, as demonstrated with a fluorescent probe. The small-angle X-ray scattering confirmed that the complex had an effect on the DNA secondary structure. With tetrahydrocortisol serving as a ligand, about 54 apolipoprotein A-I molecules were bound to a DNA molecule to break the hydrogen bonds between the heterocyclic base pairs. We suggest that a new biochemical mechanism underlies the cooperative effect of the high-density lipoproteins and Cortisol in the regulation of gene expression in hepatocytes, which also involves resident liver macrophages. This mechanism implies DNA interaction with the (apolipoprotein A-I)-tetrahydrocortisol complex, appearance of single-stranded DNA regions in the binding sites, and subsequent initiation of gene transcription.
Cite:
Panin L.E.
, Tuzikov F.V.
, Tuzikova N.A.
, Gimautdinova O.I.
, Polyakov L.M.
Interaction of Eukaryotic DNA with Apolipoprotein A-I and Its Complexes with Glucocorticoids
Biophysics. 2000. V.45. N4. P.594-602. РИНЦ
Interaction of Eukaryotic DNA with Apolipoprotein A-I and Its Complexes with Glucocorticoids
Biophysics. 2000. V.45. N4. P.594-602. РИНЦ
Original:
Panin L.E.
, Tuzikov F.V.
, Tuzikova N.A.
, Gimautdinova O.I.
, Polyakov L.M.
Interaction of Eukaryotic DNA with Apolipoprotein A-I and Its Complexes with Glucocorticoids
Биофизика. 2000. V.45. N4. P.618-619. РИНЦ
Interaction of Eukaryotic DNA with Apolipoprotein A-I and Its Complexes with Glucocorticoids
Биофизика. 2000. V.45. N4. P.618-619. РИНЦ
Identifiers:
Elibrary | 22589361 |
Citing:
DB | Citing |
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Elibrary | 1 |