Interaction of Tetrahydrocortisol–Apolipoprotein A-I Complex with Eukaryotic DNA and with Single-Stranded Oligonucleotides Full article
Journal |
Molecular Biology
ISSN: 0026-8933 , E-ISSN: 1608-3245 |
||||
---|---|---|---|---|---|
Output data | Year: 2002, Volume: 36, Number: 1, Pages: 76-81 Pages count : 6 DOI: 10.1023/A:1014254725061 | ||||
Tags | Apolipoprotein A-I, Cortisol, DNA, Lipoproteins, Oligonucleotides, Small-angle X-ray scattering, Tetrahydrocortisol | ||||
Authors |
|
||||
Affiliations |
|
Funding (1)
1 | Russian Foundation for Basic Research | 00-04-49261 |
Abstract:
The complex formed by tetrahydrocortisol (THC) and apolipoprotein A-I (ApoAI) specifically
interacts with eukaryotic DNA from rat liver. Taken together, physical and chemical data and the results of
small-angle X-ray scattering analysis show that interaction of the THC–ApoAI complex with eukaryotic DNA
results in deformation of the DNA double helix. Single-stranded fragments were demonstrated to cause deformation
of the double helix. In this state DNA forms complexes with DNA-dependent RNA polymerase. This
interaction is cooperative and of saturating type; up to six enzyme molecules bind with one DNA molecule. The
putative site of complex binding with DNA is the sequence CC(GCC)n found in many genes including the
human ApoAI gene. An oligonucleotide of this type was synthesized. Its association constant (Ka) was
1.66·106 M–1. Substitution of THC with cortisol considerably decreases the Ka. We suggest that THC interacting
with GC pairs of the binding site forms hydrogen bonds with cytosine, inducing rupture of the bonds within
the complementary nucleic base pair
Cite:
Panin L.E.
, Tuzikov F.V.
, Tuzikova N.A.
, Gimautdinova O.I.
, Polyakov L.M.
Interaction of Tetrahydrocortisol–Apolipoprotein A-I Complex with Eukaryotic DNA and with Single-Stranded Oligonucleotides
Molecular Biology. 2002. V.36. N1. P.76-81. DOI: 10.1023/A:1014254725061 WOS Scopus РИНЦ
Interaction of Tetrahydrocortisol–Apolipoprotein A-I Complex with Eukaryotic DNA and with Single-Stranded Oligonucleotides
Molecular Biology. 2002. V.36. N1. P.76-81. DOI: 10.1023/A:1014254725061 WOS Scopus РИНЦ
ArticleLinkType.TRANSLATED_TO_ORIGINAL:
Панин Л.Е.
, Тузиков Ф.В.
, Тузикова Н.А.
, Гимаутдинова О.И.
, Поляков Л.М.
Взаимодействие комплекса тетрагидрокортизол-аполипопротеин А-I с эукариотической ДНК и одноцепочечными олигонуклеотидами
Молекулярная биология. 2002. Т.36. №1. С.96-102. РИНЦ
Взаимодействие комплекса тетрагидрокортизол-аполипопротеин А-I с эукариотической ДНК и одноцепочечными олигонуклеотидами
Молекулярная биология. 2002. Т.36. №1. С.96-102. РИНЦ
Dates:
Submitted: | Feb 2, 2000 |
Published print: | Jan 1, 2002 |
Identifiers:
Web of science | WOS:000174112500013 |
Scopus | 2-s2.0-0036102335 |
Elibrary | 13392046 |
OpenAlex | W269994299 |