Selection of the Porous Layer Open Tubular Columns for Separation of Light Components in Comprehensive Two-Dimensional Gas Chromatography
Journal of Chromatography A
, E-ISSN: 1873-3778
||Comprehensive two-dimensional gas chromatography; PLOT columns; Porous polymers; Poly-(1-trimethylsilyl-1-propyne); Light components separation; Orthogonality
Patrushev Yuri V.
Sidelnikov Vladimir N.
Boreskov Institute of Catalysis, pr. Lavrentieva 5, Novosibirsk, 630090, Russia
Novosibirsk State University, Pirogova Str., 2, Novosibirsk, 630090, Russia
Ministry of Science and Higher Education of the Russian Federation
Up to the present, comprehensive two-dimensional gas chromatography was commonly performed using combinations of columns with stationary liquid phases. There are singular examples with a porous layer open tubular (PLOT) column used only in the second dimension. However, GC × GC systems with two PLOT columns are not reported in the literature. Our work describes the application of two PLOT columns in the GC × GC mode. In the first and second dimensions we used columns with the sorbents based on organic porous polymers with different selectivity: Rt-Q-BOND (nonpolar column consist of 100% divinylbenzene-styrene), Rt-S-BOND (intermediate polar column, which contain 4-vinylpyridine), Rt-U-BOND (polar column based on divinylbenzene - ethylene glycol dimethylacrylate copolymer), DVB-VIm (divinylbenzene-vinylimidazole copolymer), a column PTMSP with poly-(1-trimethylsilyl-1-propyne) sorbent, and a GASPRO column with porous silica as a stationary phase.
The degree of orthogonality was calculated for five different combinations of columns, where a column with the porous polymer was used in the first dimension, and GASPRO column – in the second dimension. Orthogonality was estimated from the correlation coefficient of retention times in the first and second dimensions.
Examples of the separation of C1-C5 hydrocarbons on a combination of PTMSP - GASPRO and Rt-Q-BOND - GASPRO columns are reported. It is shown that not only light hydrocarbons but also compounds belonging to different chemical classes can be separated with the use of PLOT columns.