DNA and Oligosaccharides Stimulate Oligomerization of Human Milk Lactoferrin Full article
Journal |
Molecular Biology
ISSN: 0026-8933 , E-ISSN: 1608-3245 |
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Output data | Year: 2009, Volume: 43, Number: 1, Pages: 142-149 Pages count : 8 DOI: 10.1134/S0026893309010191 | ||||||
Tags | ATP, DNA, Effects on oligomerization rate and degree, Human lactoferrin | ||||||
Authors |
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Affiliations |
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Funding (4)
1 | The Ministry of Education and Science of the Russian Federation | 2.2.2.3.18036 |
2 | Президиум РАН | 10.5 |
3 | Президиум РАН | 11.8 |
4 | Civilian Research and Development Foundation | Y5-B-08-11 |
Abstract:
Lactoferrin (LF), the glycoprotein transferring Fe+ ions, is contained in barrier liquids, human blood and milk. LF is an acute phase protein and one of the most important factors of nonspecific defense. The protein has unique set of biological functions. Using the methods of small-angle X-ray scattering and light-scattering it was shown for the first time that addition of DNA and oligosaccharides to LF with different level of initial oligomerization leads to an enhance in the oligomerization rate. 1M NaCl stimulates almost a complete dissociation of LF oligomeric complexes obtained in the pesence of DNA, oligosaccharides, or early founded oligomerization effectors (nucleotides). It was shown that LF oligomeric complexes obtained in the presence of different oligomerization effectors have different stability. Incubation with 50 mM MgCl2 leads to complete destruction of the protein complexes formed by ATP and oligosaccharide but partially dissociate the complexes with following formation of new in the case of AMP- and d(pT)10-dependent associates, which possess higher stability i n presence of the salt. A possible role of LF oligomerization for different biological functions of the protein is discussed.
Cite:
Soboleva S.E.
, Tuzikov F.V.
, Tuzikova N.A.
, Buneva V.N.
, Nevinsky G.A.
DNA and Oligosaccharides Stimulate Oligomerization of Human Milk Lactoferrin
Molecular Biology. 2009. V.43. N1. P.142-149. DOI: 10.1134/S0026893309010191 WOS Scopus РИНЦ
DNA and Oligosaccharides Stimulate Oligomerization of Human Milk Lactoferrin
Molecular Biology. 2009. V.43. N1. P.142-149. DOI: 10.1134/S0026893309010191 WOS Scopus РИНЦ
Original:
Соболева (Бабина) С.Е.
, Тузиков Ф.В.
, Тузикова Н.А.
, Бунева В.Н.
, Невинский Г.А.
ДНК и олигосахариды стимулируют олигомеризацию лактоферрина из молока человека
Молекулярная биология. 2009. Т.43. №1. С.157-165. Scopus РИНЦ
ДНК и олигосахариды стимулируют олигомеризацию лактоферрина из молока человека
Молекулярная биология. 2009. Т.43. №1. С.157-165. Scopus РИНЦ
Dates:
Submitted: | Apr 10, 2008 |
Accepted: | Jun 10, 2008 |
Published print: | Feb 1, 2009 |
Published online: | Feb 25, 2009 |
Identifiers:
Web of science | WOS:000263684800019 |
Scopus | 2-s2.0-61349134917 |
Elibrary | 13598604 |
Chemical Abstracts | 2009:231708 |
Chemical Abstracts (print) | 150:465898 |
OpenAlex | W2066179656 |