Comparative Analysis of Interaction of Human and Yeast DNA Damage Recognition Complexes with Damaged DNA in Nucleotide Excision Repair Full article
Journal |
Journal of Biological Chemistry
ISSN: 0021-9258 , E-ISSN: 1083-351X |
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Output data | Year: 2013, Volume: 288, Number: 15, Pages: 10936-10947 Pages count : 12 DOI: 10.1074/jbc.m112.444026 | ||||||||
Tags | Binding energy; Electrophoretic mobility; Nucleotides; Proteins; Repair; Yeast | ||||||||
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Abstract:
The human XPC-RAD23B complex and its yeast ortholog, Rad4-Rad23, are the primary initiators of global genome nucleotide excision repair. The interaction of these proteins with damaged DNA was analyzed using model DNA duplexes containing a single fluorescein-substituted dUMP analog as a lesion. An electrophoretic mobility shift assay revealed similarity between human and yeast proteins in DNA binding. Quantitative analyses of XPC/Rad4 binding to the model DNA structures were performed by fluorescent depolarization measurements. XPC-RAD23B and Rad4-Rad23 proteins demonstrate approximately equal binding affinity to the damaged DNA duplex (KD ∼ (0.5 ± 0.1) and (0.6 ± 0.3) nM, respectively). Using photoreactive DNA containing 5-iodo-dUMP in defined positions, XPC/Rad4 location on damaged DNA was shown. Under conditions of equimolar binding to DNA both proteins exhibited the highest level of cross-links to 5I-dUMP located exactly opposite the damaged nucleotide. The positioning of the XPC and Rad4 proteins on damaged DNA by photocross-linking footprinting is consistent with x-ray analysis of the Rad4-DNA crystal complex. The identity of the XPC and Rad4 location illustrates the common principles of structure organization of DNA damage-scanning proteins from different Eukarya organisms.
Cite:
Krasikova Y.S.
, Rechkunova N.I.
, Maltseva E.A.
, Pestryakov P.E.
, Petruseva I.O.
, Sugasawa K.
, Chen X.
, Min J-H.
, Lavrik O.I.
Comparative Analysis of Interaction of Human and Yeast DNA Damage Recognition Complexes with Damaged DNA in Nucleotide Excision Repair
Journal of Biological Chemistry. 2013. V.288. N15. P.10936-10947. DOI: 10.1074/jbc.m112.444026 WOS Scopus РИНЦ
Comparative Analysis of Interaction of Human and Yeast DNA Damage Recognition Complexes with Damaged DNA in Nucleotide Excision Repair
Journal of Biological Chemistry. 2013. V.288. N15. P.10936-10947. DOI: 10.1074/jbc.m112.444026 WOS Scopus РИНЦ
Files:
Full text from publisher
Dates:
Submitted: | Dec 11, 2012 |
Accepted: | Feb 7, 2013 |
Published online: | Feb 26, 2013 |
Published print: | Apr 12, 2013 |
Identifiers:
Web of science | WOS:000317565000070 |
Scopus | 2-s2.0-84876248609 |
Elibrary | 20432991 |
Chemical Abstracts | 2013:590072 |
Chemical Abstracts (print) | 158:572183 |
OpenAlex | W2023035457 |