Efficient Inhibition of Human Influenza A Virus by Oligonucleotides Electrostatically Fixed on Polylysine-Containing TiO2 Nanoparticles Full article
Journal |
Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620 , E-ISSN: 1608-330X |
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Output data | Year: 2014, Volume: 40, Number: 2, Pages: 179-184 Pages count : 6 DOI: 10.1134/S1068162014020095 | ||||||
Tags | DNA fragments, influenza A virus, nanocomposites, nanoparticles, site-specific action | ||||||
Authors |
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Affiliations |
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Funding (3)
1 | Russian Foundation for Basic Research | 11-04-01408 (01201158854) |
2 | The Ministry of Education and Science of the Russian Federation | 16.512.11.2267 (01201176480) |
3 | Siberian Branch of the Russian Academy of Sciences | 61 |
Abstract:
Antiviral activity of the TiO2·PL·DNA nanobiocomposites was studied on the MDCK cell culture infected with influenza A virus (subtype H3N2). DNA fragments in the nanocomposites are electrostatically bound to titanium dioxide nanoparticles precovered with polylysine. It was shown that TiO2·PL·DNA(v3′) nanocomposite bearing the DNA(v3′) fragment targeted to the 3′-end of the noncoding region of segment 5 of viral RNA specifically inhibited the virus reproduction with the efficiency of 99.8% and 99.9% (i.e., by factors of ∼400 and 1000, respectively) at a low concentration of DNA(v3′) in nanocomposite (0.1 and 0.2 μM, respectively). The TiO2·PL·DNA(r) nanocomposite containing an oligonucleotide noncomplementary to viral RNA or oligonucleotide DNA(v3′) unbound to the nanoparticles show very low antiviral activity (inhibition by factors of ∼3.5 and 1.3, respectively).
Cite:
Levina A.S.
, Repkova M.N.
, Ismagilov Z.R.
, Shikina N.V.
, Mazurkova N.A.
, Zarytova V.F.
Efficient Inhibition of Human Influenza A Virus by Oligonucleotides Electrostatically Fixed on Polylysine-Containing TiO2 Nanoparticles
Russian Journal of Bioorganic Chemistry. 2014. V.40. N2. P.179-184. DOI: 10.1134/S1068162014020095 WOS Scopus РИНЦ
Efficient Inhibition of Human Influenza A Virus by Oligonucleotides Electrostatically Fixed on Polylysine-Containing TiO2 Nanoparticles
Russian Journal of Bioorganic Chemistry. 2014. V.40. N2. P.179-184. DOI: 10.1134/S1068162014020095 WOS Scopus РИНЦ
Original:
Левина А.С.
, Репкова М.Н.
, Исмагилов З.Р.
, Шикина Н.В.
, Мазуркова Н.А.
, Зарытова В.Ф.
Эффективное ингибирование вируса Гриппа А человека олигонуклеотидами, электростатически фиксированными на полилизинсодержащих TiO2-наночастицах
Биоорганическая химия. 2014. Т.40. №2. С.196-202. DOI: 10.7868/S0132342314020092 Scopus РИНЦ
Эффективное ингибирование вируса Гриппа А человека олигонуклеотидами, электростатически фиксированными на полилизинсодержащих TiO2-наночастицах
Биоорганическая химия. 2014. Т.40. №2. С.196-202. DOI: 10.7868/S0132342314020092 Scopus РИНЦ
Dates:
Submitted: | Aug 14, 2013 |
Accepted: | Oct 14, 2013 |
Published print: | Mar 1, 2014 |
Published online: | Mar 26, 2014 |
Identifiers:
Web of science | WOS:000336053700008 |
Scopus | 2-s2.0-84897448416 |
Elibrary | 21870111 |
Chemical Abstracts | 2014:486110 |
Chemical Abstracts (print) | 162:85809 |
OpenAlex | W1985007921 |