The Influence of Repeated Administration of Poloxamer 407 on Serum Lipoproteins and Protease Activity in Mouse Liver and Heart Full article
Journal |
Canadian Journal of Physiology and Pharmacology
ISSN: 0008-4212 , E-ISSN: 1205-7541 |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Output data | Year: 2012, Volume: 90, Number: 11, Pages: 1456-1468 Pages count : 13 DOI: 10.1139/y2012-118 | ||||||||||
Tags | Cathepsins B, L and D, Chitotriosidase, Lipoprotein fractions and subfractions, Matrix metalloproteases, P-407-induced heart and liver injury | ||||||||||
Authors |
|
||||||||||
Affiliations |
|
Funding (1)
1 | Siberian Branch of the Russian Academy of Sciences |
Abstract:
The effects of repeated administration of poloxamer 407 (P-407) on lipoprotein-cholesterol (LP-C) and lipoprotein-triglyceride (LP-TG) fractions and subfractions, as well as the effect on liver and heart proteases, were studied. Repeated administration of P-407 to male CBA mice resulted in a model of atherosclerosis with increased diastolic blood pressure; there was a drastic increase in total serum cholesterol and especially TG. A novel small-angle X-ray scattering method for the determination of the fractional and subfractional composition of LP-C and LP-TG was used. In chronically P-407-treated mice, P-407 significantly increased atherogenic low-density lipoprotein C (LDL-C) fractions, as well as intermediate-density lipoprotein C (IDL-C), and LDL₁₋₃-C subfractions, and very-low-density lipoprotein-C (VLDL-C) fractions, as well as VLDL₁₋₂-C and VLDL₃₋₅-C subfractions), to a lesser extent, the total anti-atherogenic high-density lipoprotein C (HDL-C) fraction, as well as HDL₂-C and HDL₃-C subfractions. Additionally, we demonstrated an increase in the serum chitotriosidase activity, without significant changes in serum matrix metalloprotease (MMP) activity. Morphological changes observed in P-407-treated mice included atherosclerosis in the heart and storage syndrome in the liver macrophages. P-407 significantly increased the activity of cysteine, aspartate proteases, and MMPs in the heart, and only the activity of cathepsin B and MMPs in the liver of mice. Thus, repeated administration of P-407 to mice induced atherosclerosis secondary to sustained dyslipidemia and formation of foamy macrophages in liver, and also modulated the activity of heart and liver proteases.
Cite:
Korolenko T.A.
, Tuzikov F.V.
, Johnston T.P.
, Tuzikova N.A.
, Kisarova Y.A.
, Zhanaeva S.Y.
, Alexeenko T.V.
, Zhukova N.A.
, Brak I.V.
, Spiridonov V.K.
, Filjushina E.E.
, Cherkanova M.S.
, Monoszon A.A.
The Influence of Repeated Administration of Poloxamer 407 on Serum Lipoproteins and Protease Activity in Mouse Liver and Heart
Canadian Journal of Physiology and Pharmacology. 2012. V.90. N11. P.1456-1468. DOI: 10.1139/y2012-118 WOS Scopus РИНЦ
The Influence of Repeated Administration of Poloxamer 407 on Serum Lipoproteins and Protease Activity in Mouse Liver and Heart
Canadian Journal of Physiology and Pharmacology. 2012. V.90. N11. P.1456-1468. DOI: 10.1139/y2012-118 WOS Scopus РИНЦ
Dates:
Submitted: | Mar 23, 2012 |
Accepted: | Jun 29, 2012 |
Published print: | Nov 1, 2012 |
Published online: | Nov 13, 2012 |
Identifiers:
Web of science | WOS:000311728500004 |
Scopus | 2-s2.0-84870187731 |
Elibrary | 19103037 |
Chemical Abstracts | 2012:1666298 |
Chemical Abstracts (print) | 158:644875 |
OpenAlex | W2142136222 |