Electrical and Viscoelastic Parameters of Erythrocytes in Combination with the Fatty Acid Profile of Their Membranes and Serum as Potential Biomarkers for the Diagnosis of Colorectal Cancer
27th United European Gastroenterology Week 2019
19-23 Oct 2019
ConferenceType.INTERNATIONAL_CONFERENCE, Barcelona, Spain
|| Kruchinina M. V.
, Gromov Andrei Aleksandrovich
, Prudnikova Ya. I.
, Kurilovich Svetlana Arsentʹevna
, Kruchinin Vladimir Nikolaevich
, Generalov Vladimir Mikhailovich
, Shashkov Mikhail Vadimovich
, Sokolova Anastasiya Sergeevna
, Yakovina I. N.
, Bannova N. A.
, Shuvalov G. V.
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
Rzhanov Institute of Semiconductor Physics SB RAS
State Research Center of Virology and Biotechnology "VECTOR"
Boreskov Institute of Catalysis SB RAS
Vorozhtzov Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Science
Novosibirsk State Technical University
Center of Instrument Metrology
The aim of the study was to study the diagnostic efficiency of using the electric and viscoelastic parameters of red blood cells (RBC) in combination with the fatty acid profile (FA) of their membranes and serum (BS) for early diagnosis and evaluation of tumor progression in patients with colorectal cancer (CRC).
The CRC patients (62,7+8,6 years old) included 65 patients with early stages (TNM staging I, II) and 64 with late stages (TNM staging III, IV) and different tumor locations. The healthy controls (n=35, 61,7+7,5 years old) were selected by routine clinical examination. Electric, viscoelastic parameters of RBC were studied by dielectrophoresis, RBC membrane and BS FA composition were measured using GC/MS system triple quad Agilent 7000B (USA).
In the patients with CRC RBC tended to have much lower findings of the range of deformation, dipole moment, polarizability at 106 Hz, capacity of membranes and much higher levels of summarized findings of viscosity, rigidity, indices of destruction, aggregation and conductivity as compared to the ones in healthy subjects under study (p<0.0001-0.02).
The diagnostic panel containing proportion of deformed cells, the amplitude of RBC deformation at a frequency of 106 Hz, the summarized rigidity, electric conductivity - achieved high diagnostic accuracy (0.97) with AUC of 0.95, a sensitivity of 0.97 and a specificity of 0.93 for differentiating early stage patients from healthy controls.
A total of 21 differentially expressed FA in RBC membrane and BS were identified. Each FA class demonstrated specific changing trends in CRC progression (down-regulation in saturated FA, monounsaturated FA and up-regulation in polyunsaturated FA during cancer progression) (p<0.0001-0.001). The combination of the above RBC electrical and viscoelastic parameters and the list of FA, including С18:1;c9, C20:2n-6, C20:3n-6, C20:4n-6, C22:4n-6, C22:5n-3, C22:6n-3 - showed the best predictive power when comparing the early stages CRC patients and late ones (AUC 0.80, diagnostic accuracy 0.77, sensitivity 0.76, specificity 0.78).