ESEEM Measurements of Local Water Concentration in D2O-Containing Spin-Labeled Systems Научная публикация
Журнал |
Applied Magnetic Resonance
ISSN: 0937-9347 , E-ISSN: 1613-7507 |
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Вых. Данные | Год: 2008, Том: 35, Номер: 1, Страницы: 73-94 Страниц : 22 DOI: 10.1007/s00723-008-0144-2 | ||||||
Ключевые слова | Biological systems; Cholesterol; Concentration (process); Deuterium; Hydrates; Molecules; Spin dynamics; Water content | ||||||
Авторы |
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Организации |
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Информация о финансировании (3)
1 | Российский фонд фундаментальных исследований | 06-03-89400 |
2 | Российский фонд фундаментальных исследований | 06-04-48021 (01201166418) |
3 | Совет по грантам Президента Российской Федерации | НШ-6271.2006.3 |
Реферат:
To calibrate electron spin echo envelope modulation (ESEEM) amplitudes with respect to the deuterium water content in spin-labeled biological systems, ESEEM of nitroxide TEMPO has been studied in frozen glassy D2O-dimethylsulfoxide mixtures of different composition. The interaction between the unpaired electron of nitroxide and the deuterium nuclei manifests itself in a cosine Fourier transform spectrum as the sum of a narrow line with the doublet quadrupole splitting and of a broad one. The narrow line arises from interaction with distant deuterium nuclei, the broad one arises from interaction with nearby nuclei belonging to nitroxide-water molecule complexes. The dependence on water concentration was found to be nonlinear for the intensity of the narrow line and close to linear for the intensity of the quadrupole doublet. Therefore, the intensity of the quadrupole doublet is suggested as a measure of concentration of free water around a spin label in biological objects. Fourier transform line shape was theoretically simulated for different model distributions of water molecules around the spin label. Simulations confirm the linear dependence of the quadrupole doublet intensity on water concentration seen in the experiment. The suggested approach was applied to analyze data for spin-labeled dipalmitoylphosphatidylcholine (DPPC) and DPPC-cholesterol D2O-hydrated model membranes. The concentration of free water near the spin-labeled fourth carbon atom along the lipid chain was estimated as 5.2 and 7.2 M for DPPC and DPPC-cholesterol membranes, respectively.
Библиографическая ссылка:
Milov A.D.
, Samoilova R.I.
, Shubin A.A.
, Grishin Y.A.
, Dzuba S.A.
ESEEM Measurements of Local Water Concentration in D2O-Containing Spin-Labeled Systems
Applied Magnetic Resonance. 2008. V.35. N1. P.73-94. DOI: 10.1007/s00723-008-0144-2 WOS Scopus РИНЦ
ESEEM Measurements of Local Water Concentration in D2O-Containing Spin-Labeled Systems
Applied Magnetic Resonance. 2008. V.35. N1. P.73-94. DOI: 10.1007/s00723-008-0144-2 WOS Scopus РИНЦ
Даты:
Поступила в редакцию: | 18 янв. 2008 г. |
Опубликована в печати: | 1 нояб. 2008 г. |
Идентификаторы БД:
Web of science | WOS:000260876000006 |
Scopus | 2-s2.0-62649120466 |
РИНЦ | 13595812 |
Chemical Abstracts | 2008:1462164 |
Chemical Abstracts (print) | 151:239265 |
OpenAlex | W2025833866 |