Biopolymer and Water Dynamics in Microbial Biofilm Extracellular Polymeric Substance Научная публикация
Журнал |
Biomacromolecules
ISSN: 1525-7797 , E-ISSN: 1526-4602 |
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Вых. Данные | Год: 2008, Том: 9, Номер: 9, Страницы: 2322-2328 Страниц : 7 DOI: 10.1021/bm800269h | ||||||||||||||
Ключевые слова | Biofilms; Biofilters; Biological materials; Biomass; Biomolecules; Carbohydrates; Diffusion; Dynamics; Magnetic resonance; Molecular dynamics; Nuclear magnetic resonance; Nuclear magnetic resonance spectroscopy; Nucleic acids; Organic acids; Organic compounds; Polymers; Quantum chemistry; Renewable energy resources; Resonance | ||||||||||||||
Авторы |
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Организации |
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Информация о финансировании (11)
1 | Президиум РАН | 5.1.1 |
2 | Сибирское отделение Российской академии наук | 11 |
3 | Российский фонд фундаментальных исследований | 07-03-12147 |
4 | Совет по грантам Президента Российской Федерации | МК-5135.2007.3 |
5 | Президиум РАН | 5.2.3 |
6 | National Science Foundation | 340709 |
7 | National Science Foundation | 0642328 |
8 | National Science Foundation | 03480076 |
9 | Civilian Research and Development Foundation | |
10 | National Institutes of Health | P20 RR16455-04 |
11 | Региональный общественный Фонд содействия отечественной науке |
Реферат:
Nuclear magnetic resonance (NMR) is a noninvasive and nondestructive tool able to access several observable quantities in biofilms such as chemical composition, diffusion, and macroscale structure and transport. Pulsed gradient spin echo (PGSE) NMR techniques were used to measure spectrally resolved biomacromolecular diffusion in biofilm biomass, extending previous research on spectrally resolved diffusion in biofilms. The dominant free water signal was nulled using an inversion recovery modification of the traditional PGSE technique in which the signal from free water is minimized in order to view the spectra of components such as the rotationally mobile carbohydrates, DNA, and proteins. Diffusion data for the major constituents obtained from each of these spectral peaks demonstrate that the biomass of the biofilm contains both a fast and slow diffusion component. The dependence of diffusion on antimicrobial and environmental challenges suggests the polymer molecular dynamics measured by NMR are a sensitive indicator of biofilm function.
Библиографическая ссылка:
Hornemann J.A.
, Lysova A.A.
, Codd S.L.
, Seumour J.D.
, Busse S.C.
, Stewart P.S.
, Brown J.R.
Biopolymer and Water Dynamics in Microbial Biofilm Extracellular Polymeric Substance
Biomacromolecules. 2008. V.9. N9. P.2322-2328. DOI: 10.1021/bm800269h WOS Scopus РИНЦ
Biopolymer and Water Dynamics in Microbial Biofilm Extracellular Polymeric Substance
Biomacromolecules. 2008. V.9. N9. P.2322-2328. DOI: 10.1021/bm800269h WOS Scopus РИНЦ
Файлы:
Полный текст от издателя
Даты:
Поступила в редакцию: | 28 февр. 2008 г. |
Принята к публикации: | 28 мая 2008 г. |
Опубликована online: | 30 июл. 2008 г. |
Опубликована в печати: | 8 сент. 2008 г. |
Идентификаторы БД:
Web of science | WOS:000259033700007 |
Scopus | 2-s2.0-52649153590 |
РИНЦ | 13591587 |
Chemical Abstracts | 2008:907565 |
Chemical Abstracts (print) | 149:261937 |
OpenAlex | W1973279447 |