Total Internal Reflection Fluorescence Microscopy for Determination of Size of Individual Immobilized Vesicles: Theory and Experiment Научная публикация
Журнал |
Journal of Applied Physics
ISSN: 0021-8979 , E-ISSN: 1089-7550 |
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Вых. Данные | Год: 2015, Том: 118, Номер: 6, Номер статьи : 064702, Страниц : 11 DOI: 10.1063/1.4928083 | ||||
Ключевые слова | NANOPARTICLE TRACKING ANALYSIS; SURFACE-PLASMON RESONANCE; MEMBRANE-ANCHORED PROTEINS; SUPPORTED LIPID-BILAYERS; UNILAMELLAR VESICLES; AMPHIPATHIC HELICES; EVANESCENT FIELD; BAR DOMAINS; CORRELATION SPECTROSCOPY; EXTRACELLULAR VESICLES | ||||
Авторы |
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Организации |
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Информация о финансировании (1)
1 | Swedish Research Council |
Реферат:
Lipid vesicles immobilized via molecular linkers at a solid support represent a convenient platform for basic and applied studies of biological processes occurring at lipid membranes. Using total internal reflection fluorescence microscopy (TIRFM), one can track such processes at the level of individual vesicles provided that they contain dyes. In such experiments, it is desirable to determine the size of each vesicle, which may be in the range from 50 to 1000 nm. Fortunately, TIRFM in combination with nanoparticle tracking analysis makes it possible to solve this problem as well. Herein, we present the formalism allowing one to interpret the TIRFM measurements of the latter category. The analysis is focused primarily on the case of unpolarized light. The specifics of the use of polarized light are also discussed. In addition, we show the expected difference in size distribution of suspended and immobilized vesicles under the assumption that the latter ones are deposited under diffusion-controlled conditions. In the experimental part of our work, we provide representative results, showing explicit advantages and some shortcomings of the use of TIRFM in the context under consideration, as well as how our refined formalism improves previously suggested approaches.
Библиографическая ссылка:
Olsson T.
, Zhdanov V.P.
, Höök F.
Total Internal Reflection Fluorescence Microscopy for Determination of Size of Individual Immobilized Vesicles: Theory and Experiment
Journal of Applied Physics. 2015. V.118. N6. 064702 :1-11. DOI: 10.1063/1.4928083 WOS Scopus РИНЦ
Total Internal Reflection Fluorescence Microscopy for Determination of Size of Individual Immobilized Vesicles: Theory and Experiment
Journal of Applied Physics. 2015. V.118. N6. 064702 :1-11. DOI: 10.1063/1.4928083 WOS Scopus РИНЦ
Файлы:
Полный текст от издателя
Даты:
Поступила в редакцию: | 15 мар. 2015 г. |
Принята к публикации: | 24 июл. 2015 г. |
Опубликована online: | 11 авг. 2015 г. |
Опубликована в печати: | 14 авг. 2015 г. |
Идентификаторы БД:
Web of science | WOS:000359798600027 |
Scopus | 2-s2.0-84939138089 |
РИНЦ | 24006300 |
Chemical Abstracts | 2015:1340595 |
Chemical Abstracts (print) | 163:317321 |
OpenAlex | W1858237335 |