Biopolymer and Water Dynamics in Microbial Biofilm Extracellular Polymeric Substance Full article
Journal |
Biomacromolecules
ISSN: 1525-7797 , E-ISSN: 1526-4602 |
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Output data | Year: 2008, Volume: 9, Number: 9, Pages: 2322-2328 Pages count : 7 DOI: 10.1021/bm800269h | ||||||||||||||
Tags | Biofilms; Biofilters; Biological materials; Biomass; Biomolecules; Carbohydrates; Diffusion; Dynamics; Magnetic resonance; Molecular dynamics; Nuclear magnetic resonance; Nuclear magnetic resonance spectroscopy; Nucleic acids; Organic acids; Organic compounds; Polymers; Quantum chemistry; Renewable energy resources; Resonance | ||||||||||||||
Authors |
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Affiliations |
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Funding (11)
1 | Президиум РАН | 5.1.1 |
2 | Siberian Branch of the Russian Academy of Sciences | 11 |
3 | Russian Foundation for Basic Research | 07-03-12147 |
4 | Council for Grants of the President of the Russian Federation | МК-5135.2007.3 |
5 | Президиум РАН | 5.2.3 |
6 | National Science Foundation | 340709 |
7 | National Science Foundation | 0642328 |
8 | National Science Foundation | 03480076 |
9 | Civilian Research and Development Foundation | |
10 | National Institutes of Health | P20 RR16455-04 |
11 | Russian Science Support Foundation |
Abstract:
Nuclear magnetic resonance (NMR) is a noninvasive and nondestructive tool able to access several observable quantities in biofilms such as chemical composition, diffusion, and macroscale structure and transport. Pulsed gradient spin echo (PGSE) NMR techniques were used to measure spectrally resolved biomacromolecular diffusion in biofilm biomass, extending previous research on spectrally resolved diffusion in biofilms. The dominant free water signal was nulled using an inversion recovery modification of the traditional PGSE technique in which the signal from free water is minimized in order to view the spectra of components such as the rotationally mobile carbohydrates, DNA, and proteins. Diffusion data for the major constituents obtained from each of these spectral peaks demonstrate that the biomass of the biofilm contains both a fast and slow diffusion component. The dependence of diffusion on antimicrobial and environmental challenges suggests the polymer molecular dynamics measured by NMR are a sensitive indicator of biofilm function.
Cite:
Hornemann J.A.
, Lysova A.A.
, Codd S.L.
, Seumour J.D.
, Busse S.C.
, Stewart P.S.
, Brown J.R.
Biopolymer and Water Dynamics in Microbial Biofilm Extracellular Polymeric Substance
Biomacromolecules. 2008. V.9. N9. P.2322-2328. DOI: 10.1021/bm800269h WOS Scopus РИНЦ
Biopolymer and Water Dynamics in Microbial Biofilm Extracellular Polymeric Substance
Biomacromolecules. 2008. V.9. N9. P.2322-2328. DOI: 10.1021/bm800269h WOS Scopus РИНЦ
Files:
Full text from publisher
Dates:
Submitted: | Feb 28, 2008 |
Accepted: | May 28, 2008 |
Published online: | Jul 30, 2008 |
Published print: | Sep 8, 2008 |
Identifiers:
Web of science | WOS:000259033700007 |
Scopus | 2-s2.0-52649153590 |
Elibrary | 13591587 |
Chemical Abstracts | 2008:907565 |
Chemical Abstracts (print) | 149:261937 |
OpenAlex | W1973279447 |