The Synthesis of Insulin Fragments and the Study of Their Physicochemical and Immune Properties | Sciact - CRIS-system of Boreskov Institute of Catalysis

The Synthesis of Insulin Fragments and the Study of Their Physicochemical and Immune Properties Full article

Journal

Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620 , E-ISSN: 1608-330X

Output data

Year: 1997, Volume: 23, Number: 12, Pages: 843-849 Pages count : 7

Tags

Immunochemistry, Insulin, Receptor binding site, Small-angle X-ray scattering, Synthetic peptides

Authors

Panin L.E. ¹ , Tuzikov F.V. ² , Poteryaeva O.N. ¹ , Maksyutov A.Z. ¹ , Tuzikova N.A. ² , Sabirov A.N. ¹

Affiliations

1	Institute of Biochemistry, Siberian Division, Russian Academy of Medical Sciences, ul. Akademika Timakova 2, Novosibirsk, 630117, Russian Federation
2	Vector State Research Center of Virology and Biotechnology, Ministry of Health and Medical Industry of Russian Federation, Novosibirsk oblast, 633159, Russian Federation

A two-chain peptide was predicted as a receptor binding site of insulin on the basis of theoretical conformational analysis. This dimeric peptide, consisting of the C-terminal A18-A21 tetrapeptide of the insulin A-chain and the C-terminal B17-B30 tetradecapeptide of the insulin B-chain connected with a disulfide bridge, was synthesized along with the C-terminal nonadecapeptide. The analysis of the aggregation state of human insulin and the synthesized linear and dimeric peptides was performed by the small-angle X-ray scattering method. Specific antibodies were produced after rabbit immunization with the dimeric peptide-BSA conjugate. The immunochemical identity of the model dimeric peptide and the corresponding fragment of the insulin molecule were shown by immunoenzyme analysis.

Panin L.E. , Tuzikov F.V. , Poteryaeva O.N. , Maksyutov A.Z. , Tuzikova N.A. , Sabirov A.N.
The Synthesis of Insulin Fragments and the Study of Their Physicochemical and Immune Properties
Russian Journal of Bioorganic Chemistry. 1997. V.23. N12. P.843-849. Scopus РИНЦ

Панин Л.Е. , Тузиков Ф.В. , Потеряева О.Н. , Максютов А.З. , Тузикова Н.А. , Сабиров А.Н.
Синтез фрагментов инсулина и изучение их физико-химических и иммунологических свойств
Биоорганическая химия. 1997. Т.23. №12. С.953-960. WOS РИНЦ ANCAN

Published print:

Dec 1, 1997

Scopus:	2-s2.0-27544511456
Elibrary:	13270136

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