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Two-Dimensional Flow Nanometry of Biological Nanoparticles for Accurate Determination of Their Size and Emission Intensity Two-Dimensional Flow Nanometry of Biological Nanoparticles for Accurate Determination of Their Size and Emission Intensity Full article

Journal Nature Communications
ISSN: 2041-1723
Output data Year: 2016, Volume: 7, Article number : 12956, Pages count : 8 DOI: 10.1038/ncomms12956
Tags EXTRACELLULAR VESICLES; TETHERED VESICLE; VIRUS ENTRY; TRACKING; MICROPARTICLES; EXTRUSION; CYTOMETRY; EXOSOMES; MOTION; WATER
Authors Block Stephan 1 , Fast Bjorn Johansson 1 , Lundgren Anders 1,2 , Zhdanov Vladimir P. 1,3 , Höök Fredrik 1
Affiliations
1 Department of Physics, Division of Biological Physics, Chalmers University of Technology, Gothenburg SE-412 96, Sweden
2 Department of Nanobiotechnology, University of Natural Resources and Life Sciences, Vienna 1190, Austria
3 Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia

Funding (3)

1 Swedish Research Council 2014-5557
2 Stiftelsen för strategisk forskning RMA11-0104
3 Knut and Alice Wallenberg Foundation 2012.0055

Abstract: Biological nanoparticles (BNPs) are of high interest due to their key role in various biological processes and use as biomarkers. BNP size and composition are decisive for their functions, but simultaneous determination of both properties with high accuracy remains challenging. Optical microscopy allows precise determination of fluorescence/scattering intensity, but not the size of individual BNPs. The latter is better determined by tracking their random motion in bulk, but the limited illumination volume for tracking this motion impedes reliable intensity determination. Here, we show that by attaching BNPs to a supported lipid bilayer, subjecting them to hydrodynamic flows and tracking their motion via surface-sensitive optical imaging enable determination of their diffusion coefficients and flow-induced drifts, from which accurate quantification of both BNP size and emission intensity can be made. For vesicles, the accuracy of this approach is demonstrated by resolving the expected radius-squared dependence of their fluorescence intensity for radii down to 15 nm
Cite: Block S. , Fast B.J. , Lundgren A. , Zhdanov V.P. , Höök F.
Two-Dimensional Flow Nanometry of Biological Nanoparticles for Accurate Determination of Their Size and Emission Intensity Two-Dimensional Flow Nanometry of Biological Nanoparticles for Accurate Determination of Their Size and Emission Intensity
Nature Communications. 2016. V.7. 12956 :1-8. DOI: 10.1038/ncomms12956 WOS Scopus РИНЦ AN PMID OpenAlex
Files: Full text from publisher
Dates:
Submitted: Apr 29, 2016
Accepted: Aug 18, 2016
Published online: Sep 23, 2016
Published print: Dec 1, 2016
Identifiers:
Web of science: WOS:000385445300002
Scopus: 2-s2.0-84988566343
Elibrary: 27579115
Chemical Abstracts: 2016:1565755
PMID: 27658367
OpenAlex: W2340944929
Citing:
DB Citing
Web of science 32
Scopus 33
Elibrary 32
OpenAlex 38
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