Dual-Wavelength Surface Plasmon Resonance for Determining the Size and Concentration of Sub-Populations of Extracellular Vesicles | Sciact - CRIS-system of Boreskov Institute of Catalysis

Dual-Wavelength Surface Plasmon Resonance for Determining the Size and Concentration of Sub-Populations of Extracellular Vesicles Full article

Journal

Analytical Chemistry
ISSN: 0003-2700 , E-ISSN: 1520-6882

Output data

Year: 2016, Volume: 88, Number: 20, Pages: 9980-9988 Pages count : 9 DOI: 10.1021/acs.analchem.6b01860

Tags

Bins; Diffusion; Particle size; Plasmons

Authors

Rupert Deborah L.M. ¹ , Shelke Ganesh V. ² , Emilsson Gustav ¹ , Claudio Virginia ¹ , Block Stephan ¹ , Lässer Cecilia ² , Dahlin Andreas ¹ , Lotvall Jan O. ² , Bally Marta ^1,3 , Zhdanov Vladimir P. ^1,4 , Höök Fredrik ¹

Affiliations

1	Department of Physics, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden
2	Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition, University of Gothenburg, SE-405 30 Gothenburg, Sweden
3	Institut Curie, Centre de Recherche, CNRS, UMR 168, Physico-Chimie Curie, F-75248 Paris, France
4	Boreskov Institute of Catalysis, Russian Academy of Sciences, 630090 Novosibirsk, Russia

Funding (3)

1	Swedish Research Council	2014-5557
2	VINNOVA
3	Göran Gustafsson Foundation

Accurate concentration determination of subpopulations of extracellular vesicles (EVs), such as exosomes, is of importance both in the context of understanding their fundamental biological role and of potentially using them as disease biomarkers. In principle, this can be achieved by measuring the rate of diffusion-limited mass uptake to a sensor surface modified with a receptor designed to only bind the subpopulation of interest. However, a significant error is introduced if the targeted EV subpopulation has a size, and thus hydrodynamic diffusion coefficient, that differs from the mean size and diffusion coefficient of the whole EV population and/or if the EVs become deformed upon binding to the surface. We here demonstrate a new approach to determine the mean size (or effective film thickness) of bound nanoparticles, in general, and EV subpopulation carrying a marker of interest, in particular. The method is based on operating surface plasmon resonance simultaneously at two wavelengths with different sensing depths and using the ratio of the corresponding responses to extract the particle size on the surface. By estimating in this way the degree of deformation of adsorbed EVs, we markedly improved their bulk concentration determination and showed that EVs carrying the exosomal marker CD63 correspond to not more than around 10% of the EV sample.

Rupert D.L.M. , Shelke G.V. , Emilsson G. , Claudio V. , Block S. , Lässer C. , Dahlin A. , Lotvall J.O. , Bally M. , Zhdanov V.P. , Höök F.
Dual-Wavelength Surface Plasmon Resonance for Determining the Size and Concentration of Sub-Populations of Extracellular Vesicles
Analytical Chemistry. 2016. V.88. N20. P.9980-9988. DOI: 10.1021/acs.analchem.6b01860 WOS Scopus РИНЦ ANCAN PMID OpenAlex

Submitted:	May 20, 2016
Accepted:	Sep 20, 2016
Published online:	Oct 4, 2016
Published print:	Oct 18, 2016

Web of science:	WOS:000385907400017
Scopus:	2-s2.0-84991829537
Elibrary:	27575341
Chemical Abstracts:	2016:1534053
Chemical Abstracts (print):	165:436744
PMID:	27644331
OpenAlex:	W2521630585

DB	Citing
Web of science	68
Scopus	73
Elibrary	69
OpenAlex	76