Human Serum Albumin in Electrospun PCL Fibers: Structure, Release, and Exposure on Fiber Surface | Sciact - CRIS-система Института катализа

Human Serum Albumin in Electrospun PCL Fibers: Structure, Release, and Exposure on Fiber Surface Научная публикация

Журнал

Polymers for Advanced Technologies
ISSN: 1042-7147 , E-ISSN: 1099-1581

Вых. Данные

Год: 2017, Том: 28, Номер: 7, Страницы: 819-827 Страниц : 9 DOI: 10.1002/pat.3984

Ключевые слова

electrospinning, fiber surface, protein release, SAXS, XPS

Авторы

Chernonosova Vera S. ^1,2 , Kvon Ren I. ³ , Stepanova Alena O. ^1,2 , Larichev Yurii V. ^3,5 , Karpenko Andrey A. ¹ , Chelobanov Boris P. ² , Kiseleva Elena V. ⁴ , Laktionov Pavel P. ^1,2

Организации

1	Meshalkin Institute of Circulation Pathology, ul. Rechkunovskaya 15, Novosibirsk 630055, Russia
2	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 8, Novosibirsk 630090, Russia
3	Boreskov Institute of Catalysis, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 5, Novosibirsk 630090, Russia
4	Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk 630090, Russia
5	Novosibirsk State University, ul. Pirogova 2, Novosibirsk 630090, Russia

Информация о финансировании (1)

Российский научный фонд

14-15-00493

Human serum albumin (HSA) introduced to the fibers produced by electrospinning from HSA and polycaprolactone (PCL) solutions in hexafluoroisopropanol has been studied in terms of its structure, release from the fibers, stability of interaction with basic polymer, accessibility for protease attack, and cellular receptors, as well as dependence of the studied parameters on the protein concentration in fibers. A limited part of the protein leaves the fibers right after soaking with water, whereas the remaining protein stays tightly bound to fibers for a long time because protein nanoparticles are tightly integrated with PCL, as shown by small-angle X-ray scattering. As has been demonstrated, the proteins leave the fibers in complexes with PCL. X-ray photoelectron spectroscopy demonstrates that the protein concentration on the fiber surface is higher than the concentration in electrospinning solution. The surface-exposed protein is recognized by cell receptors and is partially hydrolyzed by proteinase K. The data on pulse protein release, presence of PCL in the protein released from matrixes, overrepresentation of the protein on the fiber surface, and tight interaction of protein with PCL may be useful for rational design of electrospun scaffolds intended for drug delivery and tissue engineering.

Chernonosova V.S. , Kvon R.I. , Stepanova A.O. , Larichev Y.V. , Karpenko A.A. , Chelobanov B.P. , Kiseleva E.V. , Laktionov P.P.
Human Serum Albumin in Electrospun PCL Fibers: Structure, Release, and Exposure on Fiber Surface
Polymers for Advanced Technologies. 2017. V.28. N7. P.819-827. DOI: 10.1002/pat.3984 WOS Scopus РИНЦ CAPlus OpenAlex

Поступила в редакцию:	30 авг. 2016 г.
Принята к публикации:	5 нояб. 2016 г.
Опубликована online:	2 дек. 2016 г.
Опубликована в печати:	1 июл. 2017 г.

Web of science:	WOS:000403007800006
Scopus:	2-s2.0-85006272129
РИНЦ:	30980933
Chemical Abstracts:	2016:1985848
OpenAlex:	W2558838311

БД	Цитирований
Web of science	24
Scopus	27
РИНЦ	29
OpenAlex	27