The Study of the Surface Layer of 3D-Matrices for Tissue Engineering | Sciact - CRIS-system of Boreskov Institute of Catalysis

The Study of the Surface Layer of 3D-Matrices for Tissue Engineering Full article

Journal

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry
ISSN: 1990-7508 , E-ISSN: 1990-7516

Output data

Year: 2017, Volume: 11, Number: 2, Pages: 139-145 Pages count : 7 DOI: 10.1134/S1990750817020020

Tags

3D-matrices, chemical modification, electrospinning, synthetic and natural polymers, X-ray photoelectron spectroscopy

Authors

Chernonosova V.S. ^1,2 , Kvon R.I. ³ , Kiseleva E.V. ⁴ , Stepanova A.O. ^1,2 , Laktionov P.P. ^1,2

Affiliations

1	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, pr. Lavrent’eva 8, Novosibirsk, 630090 Russia
2	Meshalkin Siberian Federal Biomedical Research Center, Novosibirsk, Russia
3	Boreskov Institute of Catalysis, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
4	Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia

Funding (1)

Russian Science Foundation

14-15-00493

Matrices fabricated by electrospinning from polycaprolactone solutions with human albumin or gelatin to 1,1,1,3,3,3-hexafluoroisopropanol have been investigated. Microstructure of matriх surface was analyzed using scanning electron microscopy. Protein distribution in the surface layer was studied by modification with N-(2-hydroxyethyl)phenazine and X-ray photoelectron spectroscopy. The protein concentration in the surface layer of matrices was up to 12 times higher than in the initial solution, and the lower the protein concentration in the solution, the higher the relative protein content is on the surface of matrices. During incubation of matrices in aqueous solutions protein concentration in the surface layer decreased by less than 10% during the first 30–60 min and remained at this level for a long time (seven days). Treatment with proteinase K resulted in about one-third decrease in protein concentration in the surface layer. Thus, both methods used in this study are applicable for analysis of the surface layer of materials fabricated by electrospinning from mixtures of synthetic and natural polymers; however, X-ray photoelectron spectroscopy appears to be a more informative and convenient method.

Chernonosova V.S. , Kvon R.I. , Kiseleva E.V. , Stepanova A.O. , Laktionov P.P.
The Study of the Surface Layer of 3D-Matrices for Tissue Engineering
Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry. 2017. V.11. N2. P.139-145. DOI: 10.1134/S1990750817020020 WOS Scopus РИНЦ OpenAlex

Черноносова В.С. , Квон Р.И. , Киселева Е.В. , Степанова А.О. , Лактионов П.П.
Исследование поверхностного слоя 3D-матриксов для тканевой инженерии

Биомедицинская химия. 2017. Т.63. №1. С.32-38. DOI: 10.18097/PBMC20176301032RSCI Scopus РИНЦ AN PMID OpenAlex OpenAlex

Submitted:	Sep 14, 2016
Published print:	Apr 1, 2017
Published online:	May 24, 2017

Web of science:	WOS:000405982100007
Scopus:	2-s2.0-85019661752
Elibrary:	31036332
OpenAlex:	W2619978195

DB	Citing
Web of science	2
Scopus	2
Elibrary	2
OpenAlex	2