Catalytic Properties of Lipase Entrapped as Lysates of Recombinant Strain-Producer rEscherichia coli/lip into Nanocarbon-in-Silica Composites in the Bioconversion of Triglycerides and Fatty Acids Научная публикация
| Журнал |
Applied Biochemistry and Microbiology
ISSN: 0003-6838 , E-ISSN: 1608-3024 |
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| Вых. Данные | Год: 2018, Том: 54, Номер: 1, Страницы: 38-44 Страниц : 7 DOI: 10.1134/S000368381801009X | ||||||
| Ключевые слова | recombinant lipase; rE.coli/lip; nanocarbon-silica composites; biocatalysts; esterification | ||||||
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| Организации |
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Информация о финансировании (1)
| 1 | Федеральное агентство научных организаций России | 0303-2016-0012 (V.47.1.4.) |
Реферат:
Composite multi-component biocatalysts were prepared by entrapping lysates of a recombinant rE. coli/lip strain producing Thermomyces lanuginosus lipase into composite nanocarbon-containing matrices based on a SiO2 xerogel. The dependence of the lipase activity and operational stability on the type of the carbon component (nanotubes or nanospheres of different diameters) was studied in the bioconversion of triglycerides (hydrolysis and interesterification), as well as in the esterification of saturated fatty acids—namely, butyric (C4:0), capric (C10:0), and stearic (C18:0) acids—with isoamyl alcohol. It was shown that the biocatalytic properties were determined by both the texture parameters of the nanostructured carbon included and the type of enzymatic reaction performed. Biocatalysts without a nanocarbon component had the highest operational stability in the batch process of interesterification of sunflower oil with ethyl acetate; the half-life time was found to be 720 h at 40°C. Biocatalysts containing carbon nanotubes of ~21 nm in diameter were five to six times more active in the batch esterification process than biocatalysts without a nanocarbon component. Biocatalysts containing carbon nanotubes catalyzed the synthesis of esters in a binary organic solvent (hexane and diethyl ether) without a loss of activity for more than 500 h at 40°C.
Библиографическая ссылка:
Perminova L.V.
, Kovalenko G.A.
, Beklemishev A.B.
, Mamaev A.L.
, Pykhtina M.B.
, Rudina N.A.
Catalytic Properties of Lipase Entrapped as Lysates of Recombinant Strain-Producer rEscherichia coli/lip into Nanocarbon-in-Silica Composites in the Bioconversion of Triglycerides and Fatty Acids
Applied Biochemistry and Microbiology. 2018. V.54. N1. P.38-44. DOI: 10.1134/S000368381801009X WOS Scopus РИНЦ CAPlusCA OpenAlex
Catalytic Properties of Lipase Entrapped as Lysates of Recombinant Strain-Producer rEscherichia coli/lip into Nanocarbon-in-Silica Composites in the Bioconversion of Triglycerides and Fatty Acids
Applied Biochemistry and Microbiology. 2018. V.54. N1. P.38-44. DOI: 10.1134/S000368381801009X WOS Scopus РИНЦ CAPlusCA OpenAlex
Оригинальная:
Перминова Л.В.
, Коваленко Г.А.
, Беклемишев А.Б.
, Мамаев А.Л.
, Пыхтина М.Б.
, Рудина Н.А.
Каталитические свойства липазы, включенной в наноуглерод-силикатные матрицы в виде лизатов штамма-продуцента rEscherichia сoli/lip, в реакциях биоконверсии триглицеридов и жирных кислот
Прикладная биохимия и микробиология. 2018. Т.54. №1. С.46-54. DOI: 10.7868/S0555109918010063RSCI РИНЦ OpenAlex
Каталитические свойства липазы, включенной в наноуглерод-силикатные матрицы в виде лизатов штамма-продуцента rEscherichia сoli/lip, в реакциях биоконверсии триглицеридов и жирных кислот
Прикладная биохимия и микробиология. 2018. Т.54. №1. С.46-54. DOI: 10.7868/S0555109918010063RSCI РИНЦ OpenAlex
Даты:
| Поступила в редакцию: | 27 янв. 2017 г. |
| Опубликована в печати: | 1 янв. 2018 г. |
| Опубликована online: | 8 мар. 2018 г. |
Идентификаторы БД:
| Web of science: | WOS:000427058900006 |
| Scopus: | 2-s2.0-85043386210 |
| РИНЦ: | 35499699 |
| Chemical Abstracts: | 2018:417372 |
| Chemical Abstracts (print): | 172:529840 |
| OpenAlex: | W2792243438 |