Preparation of Stable Cross-Linked Enzyme Aggregates (CLEAs) of a Ureibacillus thermosphaericus Esterase for Application in Malathion Removal from Wastewater Full article
| Journal |
Catalysts
ISSN: 2073-4344 |
||||
|---|---|---|---|---|---|
| Output data | Year: 2018, Volume: 8, Number: 4, Article number : 154, Pages count : 19 DOI: 10.3390/catal8040154 | ||||
| Tags | molecular chaperones; esterase; Ureibacillus thermosphaericus; cross-linked enzyme aggregates; response surface methodology; malathion hydrolysis | ||||
| Authors |
|
||||
| Affiliations |
|
Funding (1)
| 1 | Russian Foundation for Basic Research | 18-38-00386 |
Abstract:
In this study, the active and stable cross-linked enzyme aggregates (CLEAs) of the thermostable esterase estUT1 of the bacterium Ureibacillus thermosphaericus were prepared for application in malathion removal from municipal wastewater. Co-expression of esterase with an E. coli chaperone team (KJE, ClpB, and ELS) increased the activity of the soluble enzyme fraction up to 200.7 ± 15.5 U mg–1. Response surface methodology (RSM) was used to optimize the preparation of the CLEA-estUT1 biocatalyst to maximize its activity and minimize enzyme loss. CLEA-estUT1 with the highest activity of 29.4 ± 0.5 U mg–1 (90.6 ± 2.7% of the recovered activity) was prepared with 65.1% (w/v) ammonium sulfate, 120.6 mM glutaraldehyde, and 0.2 mM bovine serum albumin at 5.1 h of cross-linking. The biocatalyst has maximal activity at 80 ºC and pH 8.0. Analysis of the properties of CLEA-estUT1 and free enzyme at 50–80 ºC and pH 5.0–10.0 showed higher stability of the biocatalyst. CLEA-estUT1 showed marked tolerance against a number of chemicals and high operational stability and activity in the reaction of malathion hydrolysis in wastewater (up to 99.5 ± 1.4%). After 25 cycles of malathion hydrolysis at 37 ºC, it retained 55.2 ± 1.1% of the initial activity. The high stability and reusability of CLEA-estUT1 make it applicable for the degradation of insecticides.
Cite:
Samoylova Y.V.
, Sorokina K.N.
, Piligaev A.V.
, Parmon V.N.
Preparation of Stable Cross-Linked Enzyme Aggregates (CLEAs) of a Ureibacillus thermosphaericus Esterase for Application in Malathion Removal from Wastewater
Catalysts. 2018.
V.8. N4. 154
:1-19. DOI: 10.3390/catal8040154
WOS
Scopus
РИНЦ
AN
OpenAlex
Preparation of Stable Cross-Linked Enzyme Aggregates (CLEAs) of a Ureibacillus thermosphaericus Esterase for Application in Malathion Removal from Wastewater
Catalysts. 2018.
V.8. N4. 154
:1-19. DOI: 10.3390/catal8040154
WOS
Scopus
РИНЦ
AN
OpenAlex
Files:
Full text from publisher
Dates:
| Submitted: | Mar 26, 2018 |
| Accepted: | Apr 8, 2018 |
| Published print: | Apr 11, 2018 |
| Published online: | Apr 11, 2018 |
Identifiers:
| Web of science: | WOS:000435186400031 |
| Scopus: | 2-s2.0-85045387811 |
| Elibrary: | 35543626 |
| Chemical Abstracts: | 2018:1037829 |
| OpenAlex: | W2797412097 |