Heterogeneous Biocatalysts Prepared by Immuring Enzymatic Active Components Inside Silica Xerogel and Nanocarbons-in-Silica Composites Full article
| Journal |
Catalysts
ISSN: 2073-4344 |
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| Output data | Year: 2018, Volume: 8, Number: 5, Article number : 177, Pages count : 22 DOI: 10.3390/catal8050177 | ||||
| Tags | heterogeneous biocatalysts; immuring enzymatic active components; nanocarbons-in-silica composites | ||||
| Authors |
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| Affiliations |
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Funding (1)
| 1 | Ministry of Science and Higher Education of the Russian Federation | ГЗ-2017-2020 |
Abstract:
Proprietary results on preparation and studies of whole-cell and lysates-based heterogeneous biocatalysts with different enzymatic activity were reviewed. A peculiar method was developed for preparing these biocatalysts by immuring (entrapping) enzymatic active components (EAC) inside silica (SiO2) xerogel and nanocarbons-in-silica composites. Properties of the multi-component composite biocatalysts such as enzymatic activity and operational stability were compared. The effect of the inclusion of nanocarbons such as nanotubes, nanofibers, and onion-like nanospheres with various texture, nanostructure and dispersion were thoroughly studied. With invertase-active biocatalysts, the direct correlation between an increase in the enzymatic activity of the nanocarbons-in-silica biocatalyst and efficiency of EAC adhesion on nanocarbons was observed. The steady-state invertase activity of the baker yeast lysates-based biocatalysts was determined to increase by a factor of 5–6 after inclusion of the multi-walled carbon nanotubes inside SiO2-xerogel. With lipase-active biocatalysts, the effect of the included nanocarbons on the biocatalytic properties depended significantly on the reaction type. In interesterification of oil-fat blends, the biocatalysts without any included nanocarbons demonstrated the maximal lipase activity. In esterification of fatty acids with aliphatic alcohols, the activity of the biocatalysts increased by a factor of 1.5–2 after inclusion of the aggregated multi-walled carbon nanotubes (CNTs) inside SiO2-xerogel. In the low-temperature synthesis of isopentyl esters of butyric (C4:0), capric (C10:0), and srearic (C18:0) fatty acids, the lipase-active composite CNTs-in-silica biocatalysts operated without loss of activity for more than thousand hours.
Cite:
Kovalenko G.A.
, Perminova L.V.
, Beklemishev A.B.
, Parmon V.N.
Heterogeneous Biocatalysts Prepared by Immuring Enzymatic Active Components Inside Silica Xerogel and Nanocarbons-in-Silica Composites
Catalysts. 2018.
V.8. N5. 177
:1-22. DOI: 10.3390/catal8050177
WOS
Scopus
РИНЦ
AN
OpenAlex
Heterogeneous Biocatalysts Prepared by Immuring Enzymatic Active Components Inside Silica Xerogel and Nanocarbons-in-Silica Composites
Catalysts. 2018.
V.8. N5. 177
:1-22. DOI: 10.3390/catal8050177
WOS
Scopus
РИНЦ
AN
OpenAlex
Files:
Full text from publisher
Dates:
| Submitted: | Mar 2, 2018 |
| Accepted: | Apr 20, 2018 |
| Published online: | Apr 26, 2018 |
| Published print: | May 1, 2018 |
Identifiers:
| Web of science: | WOS:000435191500006 |
| Scopus: | 2-s2.0-85048036443 |
| Elibrary: | 35753559 |
| Chemical Abstracts: | 2018:1088561 |
| OpenAlex: | W2801957661 |