Catalytical Properties of Arthrobacter nicotianae Cells, a Producer of Glucose Isomerase, Immobilized Inside Xerogel of Silicium Dioxide Full article
| Journal |
Applied Biochemistry and Microbiology
ISSN: 0003-6838 , E-ISSN: 1608-3024 |
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| Output data | Year: 2009, Volume: 45, Number: 4, Pages: 389-394 Pages count : 5 DOI: 10.1134/S0003683809040073 | ||||||
| Tags | Immobilization; Fructose; Apply Biochemistry; Monosaccharide; Immobilize Cell | ||||||
| Authors |
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| Affiliations |
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Funding (2)
| 1 | Siberian Branch of the Russian Academy of Sciences | 4.4 |
| 2 | Belarusian Republican Foundation for Fundamental Research | B07CO-011 |
Abstract:
Arthrobacter nicotianae cells, producers of glucose isomerase, were immobilized inside xerogel of silicium dioxide, and properties of the resulted heterogeneous biocatalysts were investigated in the process of isomerization of monosaccharide (glucose and fructose). The glucose isomerase activity of the resulted biocatalysts was shown to be 10 U/g, on average, taking into account the loss of the activity upon the immobilization, which amounted to 50% of the cell activity in suspension. The rate of the fructose isomerization increased linearly in the range of 55–80°C with the temperature coefficient 1.3. The biocatalysts were stable in this range; they were rapidly inactivated, however, at increasing temperature. The half-pife time of inactivation was six to seven h and five min or less at 80 and 85°C, respectively. The half-pife time of inactivation of heterogeneous biocatalysts was 50–90 h in the periodic process of isomerization of 2 M monosaccharides at 60°C in the presence of the immobilized Arthrobacter nicotianae cells.
Cite:
Perminova L.V.
, Kovalenko G.A.
, Rudina N.A.
, Sapunova L.I.
, Tamkovic I.O.
, Lobanok A.G.
Catalytical Properties of Arthrobacter nicotianae Cells, a Producer of Glucose Isomerase, Immobilized Inside Xerogel of Silicium Dioxide
Applied Biochemistry and Microbiology. 2009. V.45. N4. P.389-394. DOI: 10.1134/S0003683809040073 WOS Scopus РИНЦ ANCAN OpenAlex
Catalytical Properties of Arthrobacter nicotianae Cells, a Producer of Glucose Isomerase, Immobilized Inside Xerogel of Silicium Dioxide
Applied Biochemistry and Microbiology. 2009. V.45. N4. P.389-394. DOI: 10.1134/S0003683809040073 WOS Scopus РИНЦ ANCAN OpenAlex
Original:
Перминова Л.В.
, Коваленко Г.А.
, Рудина Н.А.
, Сапунова Л.И.
, Тамкович И.О.
, Лобанок А.Г.
Каталитические свойства клеток продуцента глюкозоизомеразы Arthrobacter nicotianae, иммобилизованных в ксерогеле диоксида кремния
Прикладная биохимия и микробиология. 2009. Т.45. №4. С.432-438. Scopus РИНЦ
Каталитические свойства клеток продуцента глюкозоизомеразы Arthrobacter nicotianae, иммобилизованных в ксерогеле диоксида кремния
Прикладная биохимия и микробиология. 2009. Т.45. №4. С.432-438. Scopus РИНЦ
Dates:
| Submitted: | Feb 5, 2008 |
| Published print: | Jul 1, 2009 |
| Published online: | Jul 10, 2009 |
Identifiers:
| Web of science: | WOS:000267884300007 |
| Scopus: | 2-s2.0-70349294199 |
| Elibrary: | 15311037 |
| Chemical Abstracts: | 2009:829606 |
| Chemical Abstracts (print): | 151:311600 |
| OpenAlex: | W2029252040 |