Adsorptive Immobilization of Enzymatic Active Substances on Alumina-Silica Foam Coated by Carbon Nanofibers Full article
| Journal |
Carbon
ISSN: 0008-6223 , E-ISSN: 1873-3891 |
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| Output data | Year: 2009, Volume: 47, Number: 2, Pages: 420-427 Pages count : 8 DOI: 10.1016/j.carbon.2008.10.019 | ||
| Tags | Adsorption; Biocatalysts; Carbon nanofibers; Cell immobilization; Electron transitions; Enzyme activity; Enzyme immobilization; Foams; Glucose; Hydrolysis; Isomerization; Isomers; Silica; Sugar (sucrose); Sulfur compounds; Temperature; Yeast | ||
| Authors |
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| Affiliations |
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Funding (1)
| 1 | Siberian Branch of the Russian Academy of Sciences | 4.4 |
Abstract:
The adsorption abilities of an alumina–silica foam coated by a layer of catalytic filamentous carbon (CFC/ceramics) were studied with respect to enzymatic active substances (EAS) - enzymes glucoamylase and invertase, intracellular compartments of baker’s yeast and whole non-growing cells of various microorganisms (Saccharomyces cerevisiae, Rhodococcus sp., Arthrobacter sp.). The activity and stability of heterogeneous biocatalysts prepared by EAS adsorption on the CFC/ceramics were studied in hydrolysis, oxidation and isomerization reactions. Glucoamylase was found to retain up to 20% of the activity of a soluble enzyme after adsorption on the CFC/ceramics. Invertase and invertase-active yeast autolysate retained practically all their activity. The enzymatic activities of non-growing cells of baker’s yeast and Rhodococcus increased after adsorption on CFC/ceramics by a factor of 1.5–1.8 and 3.7, respectively. A “glucoamylase on CFC/ceramics” biocatalyst for the hydrolysis of dextrin was shown to have high long-term stability, retaining its activity after storage for 10–12 months at ambient temperature. A “yeast autolysate on CFC/ceramics” biocatalyst for sucrose inversion retained >50% of the activity after 2–3 month storage at ambient temperature. A “non-growing bacteria Arthrobacter sp. on CFC/ceramics” biocatalyst for glucose isomerization retained half its activity after 12 h use at 70 °C.
Cite:
Kovalenko G.A.
, Perminova L.V.
, Chuenko T.V.
, Rudina N.A.
Adsorptive Immobilization of Enzymatic Active Substances on Alumina-Silica Foam Coated by Carbon Nanofibers
Carbon. 2009. V.47. N2. P.420-427. DOI: 10.1016/j.carbon.2008.10.019 WOS Scopus РИНЦ ANCAN OpenAlex
Adsorptive Immobilization of Enzymatic Active Substances on Alumina-Silica Foam Coated by Carbon Nanofibers
Carbon. 2009. V.47. N2. P.420-427. DOI: 10.1016/j.carbon.2008.10.019 WOS Scopus РИНЦ ANCAN OpenAlex
Dates:
| Submitted: | Feb 19, 2008 |
| Accepted: | Oct 13, 2008 |
| Published online: | Oct 22, 2008 |
| Published print: | Feb 1, 2009 |
Identifiers:
| Web of science: | WOS:000262558300006 |
| Scopus: | 2-s2.0-57949102009 |
| Elibrary: | 13598165 |
| Chemical Abstracts: | 2009:10750 |
| Chemical Abstracts (print): | 151:386969 |
| OpenAlex: | W2046523637 |