Non-Agglomerated Silicon–Organic Nanoparticles and Their Nanocomplexes with Oligonucleotides: Synthesis and Properties | Sciact - CRIS-system of Boreskov Institute of Catalysis

Non-Agglomerated Silicon–Organic Nanoparticles and Their Nanocomplexes with Oligonucleotides: Synthesis and Properties Full article

Journal

Beilstein Journal of Nanotechnology
ISSN: 2190-4286

Output data

Year: 2018, Volume: 9, Pages: 2516-2525 Pages count : 10 DOI: 10.3762/bjnano.9.234

Tags

antiviral effect; non-agglomerated silicon–organic nanoparticles; penetration; Si–NH2·ODN nanocomplexes

Authors

Levina Asya S. ^1,2 , Repkova Marina N. ^1,2 , Shikina Nadezhda V. ³ , Ismagilov Zinfer R. ³ , Yashnik Svetlana A. ³ , Semenov Dmitrii V. ² , Savinovskaya Yulia I. ² , Mazurkova Natalia A. ⁴ , Pyshnaya Inna A. ² , Zarytova Valentina F. ^1,2

Affiliations

1	Novosibirsk State University, ul. Pirogova 2, Novosibirsk, 630090, Russia
2	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, pr. Lavrent’eva 8, Novosibirsk, 630090, Russia
3	Institute of Catalysis, Siberian Branch of Russian Academy of Sciences, pr. Lavrent’eva 5, Novosibirsk, 630090, Russia
4	FBRI State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk region, 630559, Russia

Funding (2)

1	Russian Science Foundation	16-15-10073
2	Federal Agency for Scientific Organizations	0309-2016-0005 (АААА-А17-117020210024-8) (VI.62.1.3)

The development of efficient and convenient systems for the delivery of nucleic-acid-based drugs into cells is an urgent task. А promising approach is the use of various nanoparticles. Silica nanoparticles can be used as vehicles to deliver nucleic acid fragments into cells. In this work, we developed a method for the synthesis of silicon–organic (Si–NH2) non-agglomerated nanoparticles by the hydrolysis of aminopropyltriethoxysilane (APTES). The resulting product forms a clear solution containing nanoparticles in the form of low molecular weight polymer chains with [─Si(OH)(C3H6NH2)O─] monomer units. Oligonucleotides (ODN) were conjugated to the prepared Si–NH2 nanoparticles using the electrostatic interaction between positively charged amino groups of nanoparticles and negatively charged internucleotide phosphate groups in oligonucleotides. The Si–NH2 nanoparticles and Si–NH2·ODN nanocomplexes were characterized by transmission electron microscopy, atomic force microscopy and IR and electron spectroscopy. The size and zeta potential values of the prepared nanoparticles and nanocomplexes were evaluated. Oligonucleotides in Si–NH2·ODN complexes retain their ability to form complementary duplexes. The Si–NH2Flu nanoparticles and Si–NH2·ODNFlu nanocomplexes were shown by fluorescence microscopy to penetrate into human cells. The Si–NH2Flu nanoparticles predominantly accumulated in the cytoplasm whereas ODNFlu complexes were predominantly detected in the cellular nuclei. The Si–NH2·ODN nanocomplexes demonstrated a high antisense activity against the influenza A virus in a cell culture at a concentration that was lower than their 50% toxic concentration by three orders of magnitude.

Levina A.S. , Repkova M.N. , Shikina N.V. , Ismagilov Z.R. , Yashnik S.A. , Semenov D.V. , Savinovskaya Y.I. , Mazurkova N.A. , Pyshnaya I.A. , Zarytova V.F.
Non-Agglomerated Silicon–Organic Nanoparticles and Their Nanocomplexes with Oligonucleotides: Synthesis and Properties

Beilstein Journal of Nanotechnology. 2018. V.9. P.2516-2525. DOI: 10.3762/bjnano.9.234 WOS Scopus РИНЦ AN OpenAlex

Full text from publisher

Submitted:	Mar 15, 2018
Accepted:	Sep 10, 2018
Published print:	Sep 21, 2018
Published online:	Sep 21, 2018

Web of science:	WOS:000445405600001
Scopus:	2-s2.0-85054059280
Elibrary:	35739340
Chemical Abstracts:	2018:2078371
OpenAlex:	W2889997425

DB	Citing
Web of science	12
Scopus	16
Elibrary	20
OpenAlex	18