A Study of the Catalytic Properties of the Nitrile Hydratase Immobilized on Aluminum Oxides and CarbonContaining Adsorbents Full article
| Journal |
Applied Biochemistry and Microbiology
ISSN: 0003-6838 , E-ISSN: 1608-3024 |
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| Output data | Year: 2010, Volume: 46, Number: 4, Pages: 379-384 Pages count : 6 DOI: 10.1134/S0003683810040022 | ||||
| Tags | ENZYMES; CELLS | ||||
| Authors |
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| Affiliations |
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Funding (3)
| 1 | Russian Foundation for Basic Research | 07-04-96071 |
| 2 | Russian Foundation for Basic Research | 07-04-97617 |
| 3 | Siberian Branch of the Russian Academy of Sciences |
Abstract:
The nitrile hydratase isolated from Rhodococcus ruber strain gt1, displaying a high nitrile hydratase activity, was immobilized on unmodified aluminum oxides and carbon-containing adsorbents, including the carbon support Sibunit. The activity and operational stability of the immobilized nitrile hydratase were studied in the reaction of acrylonitrile transformation into acrylamide. It was demonstrated that an increase in the carbon content in the support led to an increase in the amount of adsorbed enzyme and, concurrently, to a decrease in its activity. The nitrile hydratase immobilized on Sibunit and carbon-containing aluminum α-oxide having a “crust” structure displayed the highest operational stability in acrylonitrile hydration. It was shown that the thermostability of adsorbed nitrile hydratase increased by one order of magnitude.
Cite:
Maksimova Y.G.
, Demakov V.A.
, Maksimov A.Y.
, Ovechkina G.V.
, Kovalenko G.A.
A Study of the Catalytic Properties of the Nitrile Hydratase Immobilized on Aluminum Oxides and CarbonContaining Adsorbents
Applied Biochemistry and Microbiology. 2010. V.46. N4. P.379-384. DOI: 10.1134/S0003683810040022 WOS Scopus РИНЦ ANCAN OpenAlex
A Study of the Catalytic Properties of the Nitrile Hydratase Immobilized on Aluminum Oxides and CarbonContaining Adsorbents
Applied Biochemistry and Microbiology. 2010. V.46. N4. P.379-384. DOI: 10.1134/S0003683810040022 WOS Scopus РИНЦ ANCAN OpenAlex
Original:
Максимова Ю.Г.
, Демаков В.А.
, Максимов А.Ю.
, Овечкина Г.В.
, Коваленко Г.А.
Каталитические свойства нитрилгидратазы, иммобилизованной на оксидах алюминия и углеродсодержащих адсорбентах
Прикладная биохимия и микробиология. 2010. Т.46. №4. С.416-421. Scopus РИНЦ
Каталитические свойства нитрилгидратазы, иммобилизованной на оксидах алюминия и углеродсодержащих адсорбентах
Прикладная биохимия и микробиология. 2010. Т.46. №4. С.416-421. Scopus РИНЦ
Dates:
| Submitted: | Jan 12, 2009 |
| Published print: | Jul 1, 2010 |
| Published online: | Jul 14, 2010 |
Identifiers:
| Web of science: | WOS:000279830200002 |
| Scopus: | 2-s2.0-77954509627 |
| Elibrary: | 15332691 |
| Chemical Abstracts: | 2010:868690 |
| Chemical Abstracts (print): | 155:32994 |
| OpenAlex: | W2060905486 |