Investigation on Macrokinetics of Heterogeneous Process of Monosaccharide Isomerization using Non-Growing Cells of a Glucoisomerase Producer Arthrobacter nicotianae Immobilized inside SiO2-Xerogel Full article
| Journal |
Applied Biochemistry and Microbiology
ISSN: 0003-6838 , E-ISSN: 1608-3024 |
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| Output data | Year: 2010, Volume: 46, Number: 7, Pages: 697-705 Pages count : 9 DOI: 10.1134/S0003683810070045 | ||||||||
| Tags | a plug-flow packed-bed reactor, Arthobacter nicotianae cells immobilized inside SiO2-xerogel, glucose/fructose isomerization | ||||||||
| Authors |
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| Affiliations |
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Funding (2)
| 1 | Siberian Branch of the Russian Academy of Sciences | 4.4 |
| 2 | Belarusian Republican Foundation for Fundamental Research | Б07CO-011 |
Abstract:
Macrokinetic peculiarites of heterogeneous process of monosaccharide (glucose/fructose) isomerization using biocatalysts prepared by incorporation of non-growing cells of a glucose isomerase-producing strain Arthobacter nicotianae inside SiO2-xerogel have been investigated. It was shown that the process proceeds in kinetic regime without diffusion limitation and biocatalyst activities at 60 and 75°C were 19 and 32 U/g, respectively. Time of equilibrium in the reaction of monosaccharide isomerization was a function of starting (“triggering”) glucose isomerase activity in a unit of reaction volume. When the activity exceeds 10 U/ml, equilibrium equimolar mixture of glucose and fructose was produced within a few hours. It was established that a continuous process carried out in a plug-flow packed-bed reactor is more efficient than a batch process accompanied with recycling, first of all, to significant improvement of operation stability of the designed biocatalysts. Under model conditions of industrial heterogeneous process of producing glucose-fructose syrups, the half-life time of inactivation of the biocatalysts was more than 500 h at (65 ± 5)°C.
Cite:
Kovalenko G.A.
, Perminova L.V.
, Chernyak E.I.
, Sapunova L.I.
Investigation on Macrokinetics of Heterogeneous Process of Monosaccharide Isomerization using Non-Growing Cells of a Glucoisomerase Producer Arthrobacter nicotianae Immobilized inside SiO2-Xerogel
Applied Biochemistry and Microbiology. 2010. V.46. N7. P.697-705. DOI: 10.1134/S0003683810070045 WOS Scopus РИНЦ ANCAN OpenAlex
Investigation on Macrokinetics of Heterogeneous Process of Monosaccharide Isomerization using Non-Growing Cells of a Glucoisomerase Producer Arthrobacter nicotianae Immobilized inside SiO2-Xerogel
Applied Biochemistry and Microbiology. 2010. V.46. N7. P.697-705. DOI: 10.1134/S0003683810070045 WOS Scopus РИНЦ ANCAN OpenAlex
Original:
Коваленко Г.А.
, Перминова Л.В.
, Черняк Е.И.
, Сапунова Л.И.
Исследование макрокинетики гетерогенного процесса изомеризации моносахаридов с участием не растущих клеток продуцента глюкозоизомеразы Arthrobacter nicotianae, иммобилизованных в SIO2 - ксерогеле
Биотехнология. 2009. №5. С.63-73. RSCI РИНЦ ANCAN
Исследование макрокинетики гетерогенного процесса изомеризации моносахаридов с участием не растущих клеток продуцента глюкозоизомеразы Arthrobacter nicotianae, иммобилизованных в SIO2 - ксерогеле
Биотехнология. 2009. №5. С.63-73. RSCI РИНЦ ANCAN
Dates:
| Submitted: | Sep 2, 2008 |
| Published online: | Nov 3, 2010 |
| Published print: | Dec 1, 2010 |
Identifiers:
| Web of science: | WOS:000284166300004 |
| Scopus: | 2-s2.0-78649310808 |
| Elibrary: | 16618768 |
| Chemical Abstracts: | 2010:1367921 |
| Chemical Abstracts (print): | 154:383268 |
| OpenAlex: | W2019872413 |