Heterogeneous Biocatalysts for Production of Sweeteners-Starch Treacle and Syrups of Different Carbohydrate Composition Full article
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Catalysis in Industry
ISSN: 2070-0504 , E-ISSN: 2070-0555 |
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| Output data | Year: 2010, Volume: 2, Number: 2, Pages: 180-185 Pages count : 6 DOI: 10.1134/S2070050410020145 | ||||
| Tags | glucoamylase, glucose isomerase activity, heterogeneous biocatalysts, immobilization, invertase activity | ||||
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Abstract:
The biocatalytic heterogeneous processes are the basis for the most up-to-date biotechnologies of starch renewable resources processing into called-for sweeteners, such as treacle and syrups. Heterogeneous biocatalysts prepared by immobilization of enzymatically active substances; in particular whole non-growing microorganisms on/inside inorganic supports were developed for the different stages of the starch processing. For starch saccharification the biocatalyst with glucoamylase activity was prepared by adsorption of the enzyme GlucoLux on the nanoporous carbon support Sibunit. The biocatalyst possessed remarkably high activity (ca.700 U/g) and stability (the half-inactivation time t 1/2 > 350 h) at 60°C. For glucose isomerisation the biocatalyst with glucose isomerase activity was prepared by inclusion of biomass of bacterial strains produced intracellular enzyme and insoluble hydroxo compounds of Co(II), which are required for enzyme stabilization, inside the silica xerogel. The stability of the biocatalyst in continuous process of glucose isomerisation was sufficiently high (t 1/2 > 500 h) at 65°C. For sucrose inversion the biocatalyst with invertase activity was prepared by inclusion of biomass of baker’s yeast inside the silica xerogel. At optimal ratio of biomass to SiO2 the invertase activity of the biocatalyst reached up to ca. 600 U/g, and t 1/2 was more than 200 h at 50°C.
Cite:
Kovalenko G.A.
, Perminova L.V.
, Sapunova L.I.
Heterogeneous Biocatalysts for Production of Sweeteners-Starch Treacle and Syrups of Different Carbohydrate Composition
Catalysis in Industry. 2010. V.2. N2. P.180-185. DOI: 10.1134/S2070050410020145 WOS Scopus РИНЦ OpenAlex
Heterogeneous Biocatalysts for Production of Sweeteners-Starch Treacle and Syrups of Different Carbohydrate Composition
Catalysis in Industry. 2010. V.2. N2. P.180-185. DOI: 10.1134/S2070050410020145 WOS Scopus РИНЦ OpenAlex
Original:
Коваленко Г.А.
, Перминова Л.В.
, Сапунова Л.И.
Гетерогенные биокатализаторы для получения сахаристых крахмалопродуктов - паток и сиропов различного углеводного состава
Катализ в промышленности. 2010. №2. С.61-67. РИНЦ ANCAN
Гетерогенные биокатализаторы для получения сахаристых крахмалопродуктов - паток и сиропов различного углеводного состава
Катализ в промышленности. 2010. №2. С.61-67. РИНЦ ANCAN
Dates:
| Submitted: | Apr 30, 2009 |
| Published print: | Jun 1, 2010 |
| Published online: | Jul 10, 2010 |
Identifiers:
| Web of science: | WOS:000433658300014 |
| Scopus: | 2-s2.0-78651569101 |
| Elibrary: | 16680177 |
| OpenAlex: | W1981956367 |