Abstract: A peculiar method for immobilization of the whole non-growing microorganisms by entrapment of microbial biomass into silica xerogel was developed. The silica hydrogel was prepared by a sol-gel procedure and then was used as a precursor for preparation of the heterogeneous biocatalysts. The optimal compositions (ratio of microbial biomass to SiO2) of the biocatalysts with the high enzymatic activity and stability were found to depend strongly on the species of microorganisms used. As an example, for bacteria Arthrobacter nicotianae the biomass content did not exceed 15 wt. % of dry-weight substances. In this case, the inactivation half-time of the biocatalyst with glucose isomerase activity was more than 500 h of continuous operation in monosaccharide isomerization at 65±5°C. As another example, the biocatalysts based on the bakers' yeast contained up to 80 wt. % of dry-weight microbial biomass, and the invertase activity of these biocatalysts reached 600 U/g at 50°C. © 2010 Nova Science Publishers, Inc.

Cite: Perminova L.V. , Kovalenko G.A. , Sapunova L.I.
A Peculiar Method for Immobilization of non-Growing Microbial Cells by Entrapment into Silica Xerogel
Monography chapter Biotechnology in Medicine, Foodstuffs, Biocatalysis, Environment and Biogeotechnology. – Nova Science Publishers, Inc., 2010. – C.41-49. WOS Scopus РИНЦ

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Web of science:	WOS:000284123600005
Scopus:	2-s2.0-85048058915
Elibrary:	38614631

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