Influence of NaCl Concentration on Bicelle-Mediated SLB Formation | Sciact - CRIS-система Института катализа

Influence of NaCl Concentration on Bicelle-Mediated SLB Formation Научная публикация

Журнал

Langmuir
ISSN: 0743-7463 , E-ISSN: 1520-5827

Вых. Данные

Год: 2019, Том: 35, Номер: 32, Страницы: 10658-10666 Страниц : 9 DOI: 10.1021/acs.langmuir.9b01644

Ключевые слова

SUPPORTED LIPID-BILAYERS; MEMBRANES; MIXTURES; VESICLES; DEFORMATION; KINETICS; ADHESION; SIZE

Авторы

Sut Tun Naw ¹ , Jackman Joshua A. ² , Yoon Bo Kyeong ¹ , Park Soohyun ¹ , Kolahdouzan Kavoos ³ , Ma Gamaliel Junren ¹ , Zhdanov Vladimir P. ⁴ , Cho Nam-Joon ^1,5

Организации

1	School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore
2	School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea
3	Department of Chemistry, Pomona College, 645 North College Avenue, Claremont, California 91711, United States
4	Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia
5	School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive 637459, Singapore

Информация о финансировании (3)

1	National Research Foundation of Singapore	NRF-CRP10-2012-07
2	National Research Foundation of Singapore	NRF2015NRF-POC0001-019
3	National Research Foundation of Korea	2016M3D1A1024098

The deposition of two-dimensional bicellar disks on hydrophilic surfaces is an emerging approach to fabricate supported lipid bilayers (SLBs) that requires minimal sample preparation, works at low lipid concentrations, and yields high-quality SLBs. While basic operating steps in the fabrication protocol mimic aspects of the conventional vesicle fusion method, lipid bicelles and vesicles have distinct architectural properties, and understanding how experimental parameters affect the efficiency of bicelle-mediated SLB formation remains to be investigated. Herein, using the quartz crystal microbalance-dissipation and localized surface plasmon resonance techniques, we investigated the effect of bulk NaCl concentration on bicelle-mediated SLB formation on silicon dioxide surfaces. For comparison, similar experiments were conducted with vesicles as well. In both cases, SLB formation was observed to occur rapidly provided that the NaCl concentration was sufficiently high (>50 mM). Under such conditions, the effect of NaCl concentration on SLB formation was minor in the case of bicelles and significant in the case of vesicles where it is expected to be related primarily to osmotic pressure. At lower NaCl concentrations, bicelles also formed SLBs but slowly, whereas adsorbed vesicles remained intact. These findings were complemented by time-lapsed fluorescence microscopy imaging and fluorescence recovery after photobleaching measurements that corroborated bicelle-mediated SLB formation across the range of tested NaCl concentrations. The results are discussed by comparing the architectural properties of bicelles and vesicles along with theoretical analysis of the corresponding adsorption kinetics.

Sut T.N. , Jackman J.A. , Yoon B.K. , Park S. , Kolahdouzan K. , Ma G.J. , Zhdanov V.P. , Cho N-J.
Influence of NaCl Concentration on Bicelle-Mediated SLB Formation
Langmuir. 2019. V.35. N32. P.10658-10666. DOI: 10.1021/acs.langmuir.9b01644 WOS Scopus РИНЦ CAPlus PMID OpenAlex

Поступила в редакцию:	31 мая 2019 г.
Принята к публикации:	16 июл. 2019 г.
Опубликована online:	18 июл. 2019 г.
Опубликована в печати:	13 авг. 2019 г.

Web of science:	WOS:000480827000045
Scopus:	2-s2.0-85071318786
РИНЦ:	41660521
Chemical Abstracts:	2019:1409440
PMID (PubMed):	31318563
OpenAlex:	W2963312799

БД	Цитирований
Web of science	27
Scopus	26
РИНЦ	25
OpenAlex	28