Реферат: Various RNAs (siRNAs, miRNAs, or mRNAs) can be delivered into cells by lipid nanoparticles (LNPs) of 50–150 nm in diameter. The subsequent RNA release from LNPs may occur via various scenarios. Herein, two related kinetic models are proposed. The first model takes into account that LNPs are often porous so that RNA molecules diffuse in and detach from nanopores. The analysis is focused on RNA diffusion from a pore. The analytical expression obtained for the RNA escape rate constant is used to identify the difference in the release of siRNAs, miRNAs, and mRNAs. The key message here is that the mRNA diffusion from pores appears to be too slow, and accordingly the mRNA release seems to occur primarily via degradation of LNPs. The second coarse-grained model describes the diffusion-mediated release of RNA from a LNP in the situation when this process is accompanied by the LNP degradation at the lipid-solution interface. The corresponding kinetics are shown in detail at different relative rates of the RNA diffusion and LNP degradation. Potentially, this can help to interpret drug plasma levels after various dosing regimens. © 2019 Elsevier B.V.

Библиографическая ссылка: Zhdanov V.P.
Intracellular RNA Delivery by Lipid Nanoparticles: Diffusion, Degradation, and Release
Biosystems. 2019. V.185. 104032 :1-8. DOI: 10.1016/j.biosystems.2019.104032 WOS Scopus РИНЦ PMID OpenAlex

Даты:

Поступила в редакцию:	13 апр. 2019 г.
Принята к публикации:	15 июл. 2019 г.
Опубликована online:	25 сент. 2019 г.
Опубликована в печати:	1 нояб. 2019 г.

Идентификаторы БД:

Web of science:	WOS:000493212900006
Scopus:	2-s2.0-85072569241
РИНЦ:	41676714
PMID (PubMed):	31563119
OpenAlex:	W2977211320

Цитирование в БД:

БД	Цитирований
Scopus	15
Web of science	16
РИНЦ	13
OpenAlex	15

Альметрики: