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Fluorescent Imaging of Polystyrene Microspheres with Cyanine Dyes for Immunofluorescence Analysis Conference Abstracts

Conference European Conferences on Biomedical Optics
23-27 Jun 2019 , Munich
Source Clinical and Preclinical Optical Diagnostics II
Compilation, SPIE. Bellingham, WA.2019. ISBN 978-151062839-7.
Journal Proceedings of SPIE - The International Society for Optical Engineering
ISSN: 0277-786X
Output data Year: 2019, Volume: 11073, Article number : 110731W, Pages count : 1 DOI: 10.1117/12.2526387
Tags Cyanine dyes; Immunofluorescence analysis; Polystyrene microspheres
Authors Shalaev Pavel V. 1 , Bondina Ekaterina V. 1 , Dolgushin Sergey A. 1 , Semeykina Victoria S. 2,3 , Sankova Natalya N. 2,3 , Parkhomchuk Ekaterina V. 2,3
Affiliations
1 National Research Univ. of Electronic Technology (Russian Federation)
2 Boreskov Institute of Catalysis (Russian Federation)
3 Novosibirsk State Univ. (Russian Federation)

Funding (1)

1 The Ministry of Education and Science of the Russian Federation 14.584.21.0021 (RFMEFI58417X0021) (АААА-А17-117101850042-0)

Abstract: Samples of liquid dispersions of 4 μm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis. © 2019 SPIE.
Cite: Shalaev P.V. , Bondina E.V. , Dolgushin S.A. , Semeykina V.S. , Sankova N.N. , Parkhomchuk E.V.
Fluorescent Imaging of Polystyrene Microspheres with Cyanine Dyes for Immunofluorescence Analysis
In compilation Clinical and Preclinical Optical Diagnostics II. – SPIE., 2019. – C.11073-67. – ISBN 978-151062839-7. DOI: 10.1117/12.2526387 WOS Scopus Scopus РИНЦ OpenAlex
Dates:
Published print: Jul 19, 2019
Identifiers:
Web of science: WOS:000502143100058
Scopus: 2-s2.0-85073749856 | 2-s2.0-85084488383
Elibrary: 41713375
OpenAlex: W2963472474
Citing: Пока нет цитирований
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