QCM-Based Measurement of Bond Rupture Forces in DNA Double Helices for Complementarity Sensing Full article
| Journal |
Langmuir
ISSN: 0743-7463 , E-ISSN: 1520-5827 |
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| Output data | Year: 2014, Volume: 30, Number: 13, Pages: 3795-3801 Pages count : 7 DOI: 10.1021/la402971a | ||||||
| Tags | SINGLE-MOLECULE; NANOPORE; ATTACHMENT; MICROSCOPY; RESOLUTION; SURFACE | ||||||
| Authors |
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| Affiliations |
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Funding (2)
| 1 | Siberian Branch of the Russian Academy of Sciences | 86 |
| 2 | The Ministry of Education and Science of the Russian Federation |
Abstract:
After fixing the DNA molecule in the form of a double helix on the surface of a thickness shear mode resonator (QCM), mechanical oscillations at increasing amplitude cause detorsion of the helix. The force necessary for detorsion can be determined from the voltage applied to the QCM at the rupture moment. The high sensitivity of this method is due to the fact that measurements are carried out in the frequency region around the QCM resonance, where any (even very weak) distortions of the consistent oscillating system cause noticeable distortions of the amplitude-frequency dependence, and these distortions are used to fix the rupture moment. The measured rupture forces were within 30–40 pN, and the sensitivity was 108 molecules. It was demonstrated that the proposed procedure allows one to determine the factors that affect the stability of the DNA double helix. This procedure can be the basis for the development of a new method of rapid DNA analysis. Experiments performed with model DNA showed that it is possible to reveal complementarity between two DNA
Cite:
Dultsev F.N.
, Kolosovsky E.A.
, Mik I.A.
, Lomzov A.A.
, Pyshnyi D.V.
QCM-Based Measurement of Bond Rupture Forces in DNA Double Helices for Complementarity Sensing
Langmuir. 2014. V.30. N13. P.3795-3801. DOI: 10.1021/la402971a WOS Scopus РИНЦ OpenAlex ANCAN
QCM-Based Measurement of Bond Rupture Forces in DNA Double Helices for Complementarity Sensing
Langmuir. 2014. V.30. N13. P.3795-3801. DOI: 10.1021/la402971a WOS Scopus РИНЦ OpenAlex ANCAN
Dates:
| Submitted: | Aug 1, 2013 |
| Accepted: | Mar 9, 2014 |
| Published online: | Mar 17, 2014 |
| Published print: | Apr 8, 2014 |
Identifiers:
| ≡ Web of science: | WOS:000334572100018 |
| ≡ Scopus: | 2-s2.0-84897982233 |
| ≡ Elibrary: | 21871714 |
| ≡ OpenAlex: | W2324443476 |
| ≡ Chemical Abstracts: | 2014:441186 |
| ≡ Chemical Abstracts (print): | 160:511433 |