RGD Peptide–Albumin Conjugate for Endothelization of Electrospun Materials | Sciact - CRIS-system of Boreskov Institute of Catalysis

RGD Peptide–Albumin Conjugate for Endothelization of Electrospun Materials Full article

Journal

Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620 , E-ISSN: 1608-330X

Output data

Year: 2019, Volume: 45, Number: 6, Pages: 793-802 Pages count : 10 DOI: 10.1134/S1068162019060116

Tags

cell adhesion receptors; electrospinning; endothelization; human serum albumin; protein modification; RGD peptide; vascular grafts

Authors

Cherepanova A.V. ^1,2 , Akisheva D. ¹ , Popova T.V. ^1,3 , Chelobanov B.P. ^1,3 , Chesalov Y.A. ⁴ , Godovikova T.S. ^1,3 , Karpenko A.A. ² , Laktionov P.P. ^1,2

Affiliations

1	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia
2	Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, 630055 Russia
3	Novosibirsk State University, Novosibirsk, 630090 Russia
4	Boreskov Institute of Catalysis, Federal Research Center, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia

Funding (2)

1	Russian Science Foundation	18-15-00080
2	Federal Agency for Scientific Organizations	0309-2016-0006 (АААА-А17-117020210026-2)

To improve the endothelization of 3D matrices produced by electrospinning we propose adding cyclo(RGDfC) peptide conjugated to human serum albumin (HSA) (HSA-c(RGDfC)) to the electrospinning solution. HSA-c(RGDfC) has been prepared using a bifunctional linker with N-hydroxysuccinimide and maleimide groups, and the product has been confirmed by 1Н NMR spectroscopy. Electrospun matrices have been fabricated from the solutions of 3% polyurethane Tecoflex EG-80A with 10% HSA and 0.01–1.3% HSA–c(RGDfC) (relative to Tec) in 1,1,1,3,3,3-hexafluoro-2-propanol. The presence of HSA and the cyclo(RGDfC) peptide on the surface of electrospun matrices has been confirmed by FTIR spectroscopy. The structure of matrices has been examined by scanning electron microscopy. The ability of the matrices to facilitate the adhesion of primary human umbilical vein endotheliocytes (HUVECs) has been studied. The presence of HSA–c(RGDfC) conjugate in the matrices enhanced cellular adhesion in a dose dependent manner with a maximum effect at 1% HSA-c(RGDfC). © 2019, Pleiades Publishing, Ltd.

Cherepanova A.V. , Akisheva D. , Popova T.V. , Chelobanov B.P. , Chesalov Y.A. , Godovikova T.S. , Karpenko A.A. , Laktionov P.P.
RGD Peptide–Albumin Conjugate for Endothelization of Electrospun Materials
Russian Journal of Bioorganic Chemistry. 2019. V.45. N6. P.793-802. DOI: 10.1134/S1068162019060116 WOS Scopus РИНЦ AN OpenAlex

Submitted:	Jun 24, 2019
Accepted:	Jul 25, 2019
Published print:	Nov 1, 2019
Published online:	Jan 16, 2020

Web of science:	WOS:000520225900040
Scopus:	2-s2.0-85078596876
Elibrary:	43241436
Chemical Abstracts:	2020:349989
OpenAlex:	W2999633149

DB	Citing
Scopus	2
Web of science	2
Elibrary	2
OpenAlex	2