Thermostable Esterase estUT1 from Ureibacillus thermosphaericus: Effect of TrxA Tag on the Enzyme Properties Full article
| Journal |
Catalysis in Industry
ISSN: 2070-0504 , E-ISSN: 2070-0555 |
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| Output data | Year: 2020, Volume: 12, Number: 2, Pages: 148-154 Pages count : 7 DOI: 10.1134/S2070050420020099 | ||
| Tags | esterase, Ureibacillus thermosphaericus, TrxA, thermostability | ||
| Authors |
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| Affiliations |
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Funding (1)
| 1 | Federal Agency for Scientific Organizations | 0303-2016-0012 (V.47.1.4.) |
Abstract:
The thermostable esterase from the bacterium Ureibacillus thermosphaericus was expressed with Trx tag from plasmid pET32b-estUT1 under T7 promoter in E. coli BL21(DE3). The specific activity and relative thermal stability of the tagged enzyme increased from 45.2 to 65.8% (1 h at 70°С). The additional TrxA tag does not affect the pH optimum of enzyme activity and substrate specificity. At the same time, the absence of the TrxA tag resulted in a significant increase in the stability of estUT1 in during incubation with various chemicals, including ethanol and methanol. The maximum catalytic efficiency (kcat/KM) for esterase was observed in the absence of the TrxA tag and was 280.0 s−1 mM−1. Thereby fusion with TrxA tag promotes the enzyme secretion in the dissolved form, but reduces its thermal stability.
Cite:
Sorokina K.N.
, Samoylova Y.V.
, Parmon V.N.
Thermostable Esterase estUT1 from Ureibacillus thermosphaericus: Effect of TrxA Tag on the Enzyme Properties
Catalysis in Industry. 2020. V.12. N2. P.148-154. DOI: 10.1134/S2070050420020099 WOS Scopus РИНЦ OpenAlex
Thermostable Esterase estUT1 from Ureibacillus thermosphaericus: Effect of TrxA Tag on the Enzyme Properties
Catalysis in Industry. 2020. V.12. N2. P.148-154. DOI: 10.1134/S2070050420020099 WOS Scopus РИНЦ OpenAlex
Original:
Сорокина К.Н.
, Самойлова Ю.В.
, Пармон В.Н.
Термостабильная эстераза estUT1 бактерии Ureibacillus thermosphaericus: влияние дополнительного процессируемого домена TrxA на свойства фермента
Катализ в промышленности. 2019. Т.19. №5. С.399-407. DOI: 10.18412/1816-0387-2019-5-399-407RSCI РИНЦ ANCAN OpenAlex
Термостабильная эстераза estUT1 бактерии Ureibacillus thermosphaericus: влияние дополнительного процессируемого домена TrxA на свойства фермента
Катализ в промышленности. 2019. Т.19. №5. С.399-407. DOI: 10.18412/1816-0387-2019-5-399-407RSCI РИНЦ ANCAN OpenAlex
Dates:
| Submitted: | May 6, 2019 |
| Accepted: | Jun 24, 2019 |
| Published print: | Apr 1, 2020 |
| Published online: | Jun 25, 2020 |
Identifiers:
| Web of science: | WOS:000543047100009 |
| Scopus: | 2-s2.0-85087060956 |
| Elibrary: | 43303009 |
| OpenAlex: | W3037878167 |