Spectrally Encoded Microspheres for Immunofluorescence Analysis Review
Journal |
Journal of Applied Polymer Science
ISSN: 0021-8995 , E-ISSN: 1097-4628 |
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Output data | Year: 2021, Volume: 138, Number: 8, Article number : 49890, Pages count : 21 DOI: 10.1002/app.49890 | ||||||||||||
Tags | bioengineering; biomedical applications; functionalization of polymers; synthesis and processing techniques | ||||||||||||
Authors |
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Affiliations |
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Funding (1)
1 | Federal Agency for Scientific Organizations | 0303-2016-0010 |
Abstract:
A modern immunofluorescence analysis based on spectrally encoded microspheres has found numerous and constantly growing applications in disease diagnosis, environmental supervision, and fundamental science. Here we present an overview of microsphere-based methods of multiplex immunofluorescence analysis and consider such important parameters of beads, that are crucial in most microsphere-based immunoassays, as size distribution, fluorescence stability, magnetic properties, and particle material. The preparation methods of the microspheres with tunable diameter, the introduction of various types of fluorochromes, and magnetic particles into the microspheres are discussed in details. This review also addresses the advantages and disadvantages of different approaches to implement technically bead-based immunofluorescence analysis. © 2020 Wiley Periodicals LLC
Cite:
Sankova N.
, Shalaev P.
, Semeykina V.
, Dolgushin S.
, Odintsova E.
, Parkhomchuk E.
Spectrally Encoded Microspheres for Immunofluorescence Analysis
Journal of Applied Polymer Science. 2021.
V.138. N8. 49890
:1-21. DOI: 10.1002/app.49890
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Spectrally Encoded Microspheres for Immunofluorescence Analysis

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Dates:
Submitted: | Sep 25, 2019 |
Accepted: | Aug 25, 2020 |
Published online: | Sep 28, 2020 |
Published print: | Feb 20, 2021 |
Identifiers:
Web of science: | WOS:000573383500001 |
Scopus: | 2-s2.0-85091614537 |
Elibrary: | 45298302 |
Chemical Abstracts: | 2020:1930792 |
OpenAlex: | W3090678303 |