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Spectrally Encoded Microspheres for Immunofluorescence Analysis Review

Journal Journal of Applied Polymer Science
ISSN: 0021-8995 , E-ISSN: 1097-4628
Output data Year: 2021, Volume: 138, Number: 8, Article number : 49890, Pages count : 21 DOI: 10.1002/app.49890
Tags bioengineering; biomedical applications; functionalization of polymers; synthesis and processing techniques
Authors Sankova Natalya 1,2 , Shalaev Pavel 3,4,5 , Semeykina Viktoriya 1,2 , Dolgushin Sergey 3,4 , Odintsova Elena 6 , Parkhomchuk Ekaterina 1,2
Affiliations
1 Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russian Federation
2 Boreskov Institute of Catalysis SB RAS, Group of template synthesis, Novosibirsk, Russian Federation
3 Gamaleya Research Center of Epidemiology and Microbiology, Translational Biomedicine Laboratory, Moscow, Russian Federation
4 Aivok LLC, Moscow, Russian Federation
5 National Research University of Electronic Technology, Institute of Biomedical Systems, Moscow, Russian Federation
6 Sechenov First Moscow State Medical University, Moscow, Russian Federation

Funding (1)

1 Federal Agency for Scientific Organizations 0303-2016-0010

Abstract: A modern immunofluorescence analysis based on spectrally encoded microspheres has found numerous and constantly growing applications in disease diagnosis, environmental supervision, and fundamental science. Here we present an overview of microsphere-based methods of multiplex immunofluorescence analysis and consider such important parameters of beads, that are crucial in most microsphere-based immunoassays, as size distribution, fluorescence stability, magnetic properties, and particle material. The preparation methods of the microspheres with tunable diameter, the introduction of various types of fluorochromes, and magnetic particles into the microspheres are discussed in details. This review also addresses the advantages and disadvantages of different approaches to implement technically bead-based immunofluorescence analysis. © 2020 Wiley Periodicals LLC
Cite: Sankova N. , Shalaev P. , Semeykina V. , Dolgushin S. , Odintsova E. , Parkhomchuk E.
Spectrally Encoded Microspheres for Immunofluorescence Analysis
Journal of Applied Polymer Science. 2021. V.138. N8. 49890 :1-21. DOI: 10.1002/app.49890 WOS Scopus РИНЦ AN OpenAlex
Files: Full text from publisher
Dates:
Submitted: Sep 25, 2019
Accepted: Aug 25, 2020
Published online: Sep 28, 2020
Published print: Feb 20, 2021
Identifiers:
Web of science: WOS:000573383500001
Scopus: 2-s2.0-85091614537
Elibrary: 45298302
Chemical Abstracts: 2020:1930792
OpenAlex: W3090678303
Citing:
DB Citing
Scopus 12
Web of science 12
Elibrary 10
OpenAlex 13
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