Immobilization of a Recombinant Strain Producing Glucose Isomerase Inside SiO2-Xerogel and Properties of Prepared Biocatalysts Full article
| Journal |
Applied Biochemistry and Microbiology
ISSN: 0003-6838 , E-ISSN: 1608-3024 |
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| Output data | Year: 2011, Volume: 47, Number: 2, Pages: 151-157 Pages count : 7 DOI: 10.1134/S0003683811020074 | ||||||
| Tags | CLOSTRIDIUM-THERMOHYDROSULFURICUM; SUBSTRATE PROTECTION; CLONING; GENE | ||||||
| Authors |
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| Affiliations |
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Funding (1)
| 1 | Siberian Branch of the Russian Academy of Sciences | 96 |
Abstract:
An original method of immobilization of non-growing microorganism cells inside xerogel of silicium dioxide containing insoluble hydroxyl compounds of cobalt(II) has been developed. A recombinant strain producing glucose isomerase has been constructed on the basis of Escherichia coli with the use of a gene of Arthrobacter nicotianae. It was revealed that glucose isomerase activity and stability of biocatalysts prepared on the basis of the recombinant E. coli strain was 3–5 times greater compared with the biocatalysts prepared with the use of the donor strain A. nicotianae. Under conditions of continuous hydrolysis of 3 M fructose at 62–65°C in a fixed bed reactor, time of half-inactivation of the biocatalysts prepared from the recombinant strain and A. nicotianae was ∼60 and ∼25 days, respectively.
Cite:
Kovalenko G.A.
, Perminova L.V.
, Chuenko T.V.
, Sapunova L.I.
, Shlyakhotko E.A.
, Lobanok A.G.
Immobilization of a Recombinant Strain Producing Glucose Isomerase Inside SiO2-Xerogel and Properties of Prepared Biocatalysts
Applied Biochemistry and Microbiology. 2011. V.47. N2. P.151-157. DOI: 10.1134/S0003683811020074 WOS Scopus РИНЦ ANCAN OpenAlex
Immobilization of a Recombinant Strain Producing Glucose Isomerase Inside SiO2-Xerogel and Properties of Prepared Biocatalysts
Applied Biochemistry and Microbiology. 2011. V.47. N2. P.151-157. DOI: 10.1134/S0003683811020074 WOS Scopus РИНЦ ANCAN OpenAlex
Original:
Коваленко Г.А.
, Перминова Л.В.
, Чуенко Т.В.
, Сапунова Л.И.
, Шляхотко Е.А.
, Лобанок А.Г.
Иммобилизация рекомбинантного штамма - продуцента глюкозоизомеразы в ксерогеле диоксида кремния и свойства приготовленных биокатализаторов
Прикладная биохимия и микробиология. 2011. Т.47. №2. С.168-175. Scopus РИНЦ
Иммобилизация рекомбинантного штамма - продуцента глюкозоизомеразы в ксерогеле диоксида кремния и свойства приготовленных биокатализаторов
Прикладная биохимия и микробиология. 2011. Т.47. №2. С.168-175. Scopus РИНЦ
Dates:
| Submitted: | Jun 13, 2010 |
| Published print: | Mar 1, 2011 |
| Published online: | Mar 9, 2011 |
Identifiers:
| Web of science: | WOS:000290321000007 |
| Scopus: | 2-s2.0-79952366642 |
| Elibrary: | 16641297 |
| Chemical Abstracts: | 2011:285312 |
| Chemical Abstracts (print): | 154:608453 |
| OpenAlex: | W2058358319 |