Реферат: Background Early diagnosis of pre-cancer and early stages of colorectal cancer (CRC) is the key to a long-term survival of patients, decreasing their invalidity rate, and reduction of therapy costs. This study aims to define the list of fatty acids (FA) of erythrocyte (Er) membranes and blood serum (BS) which could serve as biomarkers of pre-cancer (adenomatous polyps), and of early stages of CRC, depending on tumor localization. Methods 65 patients with CRC of stage 1-2 (63.2±9.4 years), 25 patients with adenomatous polyps, 35 conditionally healthy subjects were included in the study. Studies of the composition of FAs of Er and BS were conducted with the help of the Agilent 7000B (USA) LC/MS system based on three quadrupoles. Results The levels of 50 FA in BS of Er were defined. Upon completion of standardization the diagnostic panel, comprising С16:1;7, C18:2 n-6, С15:0, omega-6/omega-3, C18:0, С14:0, C18:1;t9, C20:0, C12:0, C17:0, the ratio of saturated/unsaturated FA, enabled to discern the patients with adenomatous polyps from healthy subjects with AUC 0.883, specificity of 0.89, sensitivity of 0.81. The key role in differentiation of patients with 1-2 stage of CRC from the healthy belongs to omega-3 PUFA, especially С22:6n3, the total content of С20:5 n3 and С22:6 n3, as well as the sum of all omega-3 PUFA both in the Er and BS. Their increase prevails over the increase of omega-6 PUFA (С20:2 n6, С22:3 n6, С22:4 n6), especially in BS. Only in BS we revealed the highly significant increase of С20:4 n6 (p < 0,00003) in patients with CRC as compared to healthy subjects. The model, comprising the levels of the following FAs, i.e.С14:0, С15:0, С16:1;7, С18:3 n-3, C20:2 n-6, C20:3 n-6, C20:5 n-3, C22:4 n-6, C22:5 n-3, C22:6 n-3, the sum of omega-3, omega-3 (EPA+DHA), the ratio of saturated/polyunsaturated FA, secured AUC equal to 0.916 with specificity of 0.90, sensitivity of 0.95 upon differentiation of patients with 1-2 CRC stage from healthy subjects. Erythrocyte levels of С18:3 n-3, С14:0, С15:0, С20:2 n-6 and the ones of BS (С18:3 n-3, 22:6 n-3), which tended to be higher in cases of proximal tumors as compared to the distal ones (p=0.05-0.00007), became significant in differentiation of localization of early CRC. The panel, consisting of C18:2 n-6, the sum of omega-6, C18:0, the sum of PUFA, C20:4 n-6, the ration if saturated/polyunsaturated FA, saturated/unsaturated FA, С16:1;7, C20:2 n-6, C20:3 n-6, С15:0, C22:4 n-6, C20:0, C12:0, C22:6 n-3, С18:3 n-3, enable to achieve AUC, equal to 0.824, with specificity of 0.82, sensitivity of 0.86 upon differentiation of patients with adenomatous polyps from the ones with 1-2 stage of CRC. Conclusions The levels of FA of Er membranes and BS proved to be highly significant when applied as biomarkers used in diagnosis of the pre-cancer and early cancer stage, taking into account localization of the tumor. Application of these markers in clinical practice within the course of screening and modern diagnosis will serve as a perspective trend and will result in a decrease in incidence of cases and mortality rate induced by CRC.

Библиографическая ссылка: Kruchinina M. , Gromov A. , Kruchinin V. , Shashkov M. , Sokolova A. , Yakovina I. , Shestov A.
Diagnostic Potential of Erythrocyte and Serum Fatty Acids in Spotting Adenomatous Polyps and Identifying the Early Stages of Colorectal Cancer Depending on Tumor Localization
Annals of Oncology. 2021. V.32. NS3. P.S101. DOI: 10.1016/j.annonc.2021.05.070 WOS РИНЦ OpenAlex

Даты:

Опубликована в печати:	1 июл. 2021 г.
Опубликована online:	3 июл. 2021 г.

Идентификаторы БД:

Web of science:	WOS:000670004200016
РИНЦ:	46610992
OpenAlex:	W3186244134

Цитирование в БД: Пока нет цитирований

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