Lipase-Active Heterogeneous Biocatalysts for Enzymatic Synthesis of Short-Chain Aroma Esters Научная публикация
| Журнал |
Biocatalysis and Agricultural Biotechnology
ISSN: 1878-8181 |
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| Вых. Данные | Год: 2021, Том: 36, Номер статьи : 102124, Страниц : 9 DOI: 10.1016/j.bcab.2021.102124 | ||||
| Ключевые слова | Aroma synthesis; Esterification; Lipase-active biocatalysts | ||||
| Авторы |
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| Организации |
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Информация о финансировании (1)
| 1 | Министерство науки и высшего образования Российской Федерации (с 15 мая 2018) | ГЗ-2021-2023 |
Реферат:
The lipase-active heterogeneous biocatalysts were prepared by adsorptive immobilization of Thermomyces lanuginosus lipase (designated as rPichia/lip) produced by authoring recombinant Pichia pastoris strain. Biocatalysts of LipoCarb type were prepared by spontaneous adsorption of rPichia/lip on the authoring macroporous carbon aerogel. Biocatalysts of LipoSil type were prepared by forced adsorption of rPichia/lip on mesoporous silica. The prepared biocatalysts were studied carefully in the esterification of short-chain fatty acids (butyric C4, C5–C7) and aliphatic alcohols (ethanol C2, C3–C5, C8) in order to produce valuable fruity-smelling esters. Biocatalytic properties, such as enzymatic activity, substrates specificity, and operational stability, were found to depend on chemical nature of both the adsorbents and organic solvents, primarily on their polarity. When comparing the activity in the synthesis of n-butyl heptanoate, it was found that the LipoCarb biocatalysts demonstrated activity an order of magnitude higher than LipoSil type. The Michaelis-Menten kinetic constants differed by a factor of 30, e.g. 1.1·102 s−1 and 3.7 s−1, respectively. The operational stability of the lipase-active biocatalysts was sufficiently high; rPichia/lip adsorbed on carbon aerogel retained till 96% of the initial activity after 29 reaction cycles, whereas rPichia/lip adsorbed on silica completely retained the activity after 38 reaction cycles of esterification under optimal reaction conditions selected. The prepared biocatalysts of LipoCarb and LipoSil types were promising for the Green enzymatic synthesis of fragrance and aroma esters under very mild ambient conditions (20±2 °C, 1 bar).
Библиографическая ссылка:
Kovalenko G.
, Perminova L.
, Pykhtina M.
, Beklemishev A.
Lipase-Active Heterogeneous Biocatalysts for Enzymatic Synthesis of Short-Chain Aroma Esters
Biocatalysis and Agricultural Biotechnology. 2021. V.36. 102124 :1-9. DOI: 10.1016/j.bcab.2021.102124 WOS Scopus РИНЦ CAPlusCA OpenAlex
Lipase-Active Heterogeneous Biocatalysts for Enzymatic Synthesis of Short-Chain Aroma Esters
Biocatalysis and Agricultural Biotechnology. 2021. V.36. 102124 :1-9. DOI: 10.1016/j.bcab.2021.102124 WOS Scopus РИНЦ CAPlusCA OpenAlex
Даты:
| Поступила в редакцию: | 10 июн. 2021 г. |
| Принята к публикации: | 4 авг. 2021 г. |
| Опубликована online: | 8 авг. 2021 г. |
| Опубликована в печати: | 1 сент. 2021 г. |
Идентификаторы БД:
| Web of science: | WOS:000713266000003 |
| Scopus: | 2-s2.0-85112383016 |
| РИНЦ: | 47020896 |
| Chemical Abstracts: | 2022:194007 |
| Chemical Abstracts (print): | 178:5614 |
| OpenAlex: | W3189410845 |