Comparison of Dried Matrix Spots and Fabric Phase Sorptive Extraction Methods for Quantification of Highly Potent Analgesic Activity Agent (2R,4aR,7R,8aR)-4,7-dimethyl-2-(thiophen-2-yl)octahydro-2H-chromen-4-ol in Rat Whole Blood and Plasma Using LC–MS/MS | Sciact - CRIS-система Института катализа

Comparison of Dried Matrix Spots and Fabric Phase Sorptive Extraction Methods for Quantification of Highly Potent Analgesic Activity Agent (2R,4aR,7R,8aR)-4,7-dimethyl-2-(thiophen-2-yl)octahydro-2H-chromen-4-ol in Rat Whole Blood and Plasma Using LC–MS/MS Научная публикация

Журнал

Journal of Chromatography B
ISSN: 1570-0232 , E-ISSN: 1873-376X

Вых. Данные

Год: 2019, Том: 1132, Номер статьи : 121813, Страниц : 9 DOI: 10.1016/j.jchromb.2019.121813

Ключевые слова

(2R,4aR,7R,8aR)-4,7-dimethyl-2-(thiophen-2-yl)octahydro-2H-chromen-4-ol; Analgesic agent; Dried matrix spots; Fabric phase sorptive extraction; FPSE biofluid sampler; LC–MS/MS

Авторы

Lastovka Anastasiya V. ^1,2 , Rogachev Artem D. ^1,2 , Il'ina Irina V. ¹ , Kabir Abuzar ³ , Volcho Konstantin P. ^1,2 , Fadeeva Valentina P. ^1,2 , Pokrovsky Andrey G. ² , Furton Kenneth G. ³ , Salakhutdinov Nariman F. ^1,2

Организации

1	N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of Russian Academy of Sciences, Lavrent’ev ave., 9, 630090 Novosibirsk, Russia
2	Novosibirsk State University, Pirogov St., 2, 630090 Novosibirsk, Russia
3	Department of Chemistry and Biochemistry, International Forensic Research Institute, Florida International University, 11200 SW 8th St., Miami, FL 33199, USA

The methods for quantification of highly potent analgesic agent (2R,4aR,7R,8aR)-4,7-dimethyl-2- (thiophen-2-yl)octahydro-2H-chromen-4-ol in rat whole blood and plasma were developed and validated using dried matrix spots (DMS) or fabric phase sorptive extraction (FPSE) techniques in combination with LC–MS/MS. 2-Adamantylamine hydrochloride was used as an internal standard (IS). Chromatographic separation was carried out on a reversed-phase column (2.0×75 mm, 5 μm) using water containing 0.1% formic acid and methanol containing 0.1% formic acid as mobile phases in gradient mode at a flow rate of 200 μL/min. The mass spectrometric detection was performed using electrospray ionization (ESI) in positive ion mode. MRM transitions were m/z 284.5 → 137.2/157.4 for the analgesic agent and m/z 152.3 → 93.1/107.2 for IS. Calibration curves were linear within 20–5000 ng/mL in dried plasma spots (DPS) or dried blood spots (DBS) experiments. The linearity was obtained in the range of 20–5000 ng/mL and 50–5000 ng/mL for plasma-FPSE and blood-FPSE experiments, respectively. The intra- and inter-day accuracy and precision did not exceed acceptable limits. The mean extraction recovery (%) was 26 for DPS, 25 for DBS, 38 for plasma-FPSE, 31 for blood-FPSE.

Lastovka A.V. , Rogachev A.D. , Il'ina I.V. , Kabir A. , Volcho K.P. , Fadeeva V.P. , Pokrovsky A.G. , Furton K.G. , Salakhutdinov N.F.
Comparison of Dried Matrix Spots and Fabric Phase Sorptive Extraction Methods for Quantification of Highly Potent Analgesic Activity Agent (2R,4aR,7R,8aR)-4,7-dimethyl-2-(thiophen-2-yl)octahydro-2H-chromen-4-ol in Rat Whole Blood and Plasma Using LC–MS/MS
Journal of Chromatography B. 2019. V.1132. 121813 :1-9. DOI: 10.1016/j.jchromb.2019.121813 WOS Scopus РИНЦ CAPlusCA OpenAlex

Поступила в редакцию:	10 июн. 2019 г.
Принята к публикации:	21 сент. 2019 г.
Опубликована online:	21 окт. 2019 г.
Опубликована в печати:	15 нояб. 2019 г.

Web of science:	WOS:000500361600008
Scopus:	2-s2.0-85074443097
РИНЦ:	41691277
Chemical Abstracts:	2019:2132778
Chemical Abstracts (print):	174:541796
OpenAlex:	W2982061271

БД	Цитирований
Web of science	2
Scopus	2
OpenAlex	3